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For Research Use Only. Not for Use in Diagnostic Procedures.
| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| ARP2 (D85D5) Rabbit mAb | 5614 | 40 µl | 44 kDa | Rabbit IgG |
| ARP3 Antibody | 4738 | 40 µl | 47 kDa | Rabbit |
| N-WASP (30D10) Rabbit mAb | 4848 | 40 µl | 65 kDa | Rabbit |
| Diap1 Antibody | 5486 | 40 µl | 150 kDa | Rabbit |
| Diap2 Antibody | 5474 | 40 µl | 130 kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
Storage
Background
Formins are a family of large multidomain actin nucleation/polymerization proteins characterized by their catalytic FH2 domains. The mammalian diaphanous-related formin (mDia/diap) subfamily, including mDia1/diap1, mDia2/diap3 and mDia3/diap2, are effectors of Rho family small GTPases. In response to Rho, mDia/diap proteins are involved in the regulation of multiple cell functions including cytoskeletal dynamics, migration, adhesion, polarity, and cell shape (reviewed in 4,5).
mDia1/diap1 is activated by GTP-bound Rho, leading to Rho-associated kinase (ROCK)-dependent stress fiber formation (6,7). Rho activation of mDia1 has also been shown to regulate serum response factor (SRF)-dependent transcription (8), and has been implicated in human cancer phenotypes such as ras-mediated transformation, metastasis, and invasion (reviewed in 9).
mDia3/diap2, activated by the Rho family small GTPase cdc42, regulates the attachment of microtubules to the kinetochore during mitosis in mammalian cells (10).
Rho-dependent activation of mDia2/diap3 is important in assembly of the contractile ring during cytokinesis (11,12).
Background References
- Goley, E.D. and Welch, M.D. (2006) Nat. Rev. Mol. Cell Biol. 7, 713-726.
- Morita, T. et al. (2007) J. Biol. Chem. 282, 8454-8463.
- Yoo, Y. et al. (2007) J. Biol. Chem. 282, 7616-7623.
- Schönichen, A. and Geyer, M. (2010) Biochim Biophys Acta 1803, 152-63.
- Chesarone, M.A. et al. (2010) Nat Rev Mol Cell Biol 11, 62-74.
- Watanabe, N. et al. (1999) Nat Cell Biol 1, 136-43.
- Ishizaki, T. et al. (2001) Nat Cell Biol 3, 8-14.
- Copeland, J.W. and Treisman, R. (2002) Mol Biol Cell 13, 4088-99.
- Narumiya, S. et al. (2009) Cancer Metastasis Rev 28, 65-76.
- Yasuda, S. et al. (2004) Nature 428, 767-71.
- Watanabe, S. et al. (2010) Mol Biol Cell 21, 3193-204.
- Watanabe, S. et al. (2008) Mol Biol Cell 19, 2328-38.
Trademarks and Patents
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