Dab2 Antibody #8582

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Dab2 Antibody recognizes endogenous levels of total Dab2 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro222 of human Dab2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Disabled homologue 2 (Dab2) is a mitogen responsive phosphoprotein that exerts multiple functions through association with numerous proteins. Dab2 modulates signaling pathways through interactions with proteins such as Smads and TGF-β receptors (1,2), axin (3), GRB (4) and Src (5). Dab2 also serves as a cargo-specific adaptor of clathrin-mediated endocytosis via interaction with clathrin (6), AP2 (7), NPXY-containing cargo (8-10), and myosin VI (11,12). In addition, Dab2 regulates cell adhesion by directly binding integrins (13,14). The diverse functions of Dab2 enable it to coordinate cell adhesion, cell motility, membrane trafficking, and signaling. Research studies have shown Dab2 is down-regulated in a number of cancers, thereby suggesting a role as a tumor suppressor (15-17). Phosphorylation of Dab2 decreases its endocytotic function (18).

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2. Hocevar, B.A. et al. (2005) J Biol Chem 280, 25920-7.
3. Hocevar, B.A. et al. (2003) EMBO J 22, 3084-94.
4. Xu, X.X. et al. (1998) Oncogene 16, 1561-9.
5. Zhou J et al. (2003) J Biol Chem 278, 6936–41
6. Mishra, S.K. et al. (2002) EMBO J 21, 4915-26.
7. Morris, S.M. and Cooper, J.A. (2001) Traffic 2, 111-23.
8. Keyel, P.A. et al. (2006) Mol Biol Cell 17, 4300-17.
9. Maurer, M.E. and Cooper, J.A. (2006) J Cell Sci 119, 4235-46.
10. Maurer, M.E. and Cooper, J.A. (2005) J Cell Sci 118, 5345-55.
11. Morris, S.M. et al. (2002) Traffic 3, 331-41.
12. Hasson, T. (2003) J Cell Sci 116, 3453-61.
13. Huang, C.L. et al. (2006) J Cell Sci 119, 4420-30.
14. Teckchandani, A. et al. (2009) J Cell Biol 186, 99-111.
15. Mok, S.C. et al. (1998) Oncogene 16, 2381-7.
16. Anupam, K. et al. (2006) World J Gastroenterol 12, 6041-5.
17. Bagadi, S.A. et al. (2007) Breast Cancer Res Treat 104, 277-86.
18. Chetrit, D. et al. (2011) J Biol Chem 286, 5392-403.

• Data sheet (108KB)
• MSDS PDF