Upstream / Downstream
Explore pathways related to this product.
Find answers on our FAQs page.
- Additional protein information
- Analytical tools
Derlin-1 Antibody #8897
This product is discontinued
Gallery: Derlin-1 Antibody #8897
Derlin-1 Antibody recognizes endogenous levels of total Derlin-1 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with either Derlin-2 or Derlin-3.Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig, Guinea Pig, Horse
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Derlin-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Elimination of misfolded proteins from the endoplasmic reticulum (ER) occurs largely through the ER-associated degradation (ERAD) pathway and is an important physiological adaptation to ER stress. After insertion into the lumen of the ER, glycoproteins that fail to fold properly are destined for degradation. Through a process termed retro-translocation, misfolded proteins are deposited into the cytosol from the ER, where ubiquitination, deglycosylation, and proteasomal proteolysis lead to their degradation. Derlin-1 (Der1-like protein) corresponds to a homologue of yeast Der1p, a protein identified in a genetic screen for components required for the degradation of misfolded ER luminal proteins (1). Like yeast Der1p, mammalian Derlin-1 is an ER protein that is predicted to have four transmembrane segments with both the amino and carboxy termini exposed to the cytoplasmic compartment (2-4). Derlin-1 appears to be a central, evolutionarily conserved membrane component of the retro-translocation machinery associated with the ERAD pathway. Indeed, studies have shown that Derlin-1 expression is transcriptionally upregulated in response to ER stress (5-7) and associates with ER-anchored ubiquitin ligases, such as HRD1 and gp78/AMFR, via binding to p97/VCP and VCP-interacting membrane protein (VIMP) (5,8).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.