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Specificity / Sensitivity

Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb recognizes endogenous levels of Stat1 protein only when phosphorylated at Tyr701. Stat1 antibody detects endogenous levels of total Stat1 α (91kDa) and Stat1 β (84kDa) protein.


Source / Purification

Phospho-Stat1 (Tyr701) (D4A7) monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr701 of human Stat1 protein. Stat1 polyclonal antibody is produced using a synthetic peptide corresponding to a sequence of human Stat1 (Stat1 antibody). Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.


1.  Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.

2.  Durbin, J.E. et al. (1996) Cell 84, 443-50.

3.  Meraz, M.A. et al. (1996) Cell 84, 431-42.

4.  Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.

5.  Frank, D.A. (1999) Mol Med 5, 432-56.

6.  Wen, Z. et al. (1995) Cell 82, 241-50.


Entrez-Gene Id 6772
Swiss-Prot Acc. P42224


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PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
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9170
PhosphoPlus® Stat1 (Tyr701) Antibody Kit