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- Analytical tools
PhosphoPlus® Akt Activation Kit #9280
This product is discontinued
Gallery: PhosphoPlus® Akt Activation Kit #9280
7003 5 ml
Phospho-Akt (Ser473) detects endogenous levels of Akt only when phosphorylated at Ser473. Phospho-Akt (Thr308) detects endogenous levels of Akt1 protein only when phosphorylated at Thr308, detects endogenous levels of Akt2 protein when phosphorylated at Thr309, and detects endogenous levels of Akt3 protein when phosphorylated at Thr305. The Akt antibody detects endogenous levels of total Akt1, Akt2 and Akt3 proteins independent of their phosphorylation state. It does not cross-react with related kinases.
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473, Thr308, or from the carboxy-terminal sequence of mouse Akt, or residues surrounding Thr308 of human Akt1 protein.
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).
The Akt activation kit provides an economical means to evaluate the activation status of Akt at both the Ser473 and Thr308 positions as well as the total Akt protein level. The kit includes enough primary and secondary antibodies to perform ten Western mini-blot experiments.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. PhosphoPlus is a trademark of Cell Signaling Technology, Inc. LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories. U.S. Patent No. 5,675,063.