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29087
PhosphoPlus® α-Synuclein (Ser129) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® α-Synuclein (Ser129) Antibody Duet #29087

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PhosphoPlus® α-Synuclein (Ser129) Antibody Duet: Image 1

Confocal immunofluorescent analysis of mouse hippocampus using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (upper), or α-Synuclein (D37A6) XP® Rabbit mAb #4179 (lower) (green), in the presence of Phospho-α-Synuclein (Ser129) peptide (middle) or Non-phospho-α-Synuclein (Ser129) peptide (right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

PhosphoPlus® α-Synuclein (Ser129) Antibody Duet: Image 2

Confocal immunofluorescent analysis of normal rat cerebellum, hippocampus and striatum using α-Synuclein (D37A6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

PhosphoPlus® α-Synuclein (Ser129) Antibody Duet: Image 3

Confocal immunofluorescent analysis of untreated (left) or λ phosphatase-treated (right) mouse hippocampus using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (upper), or α-Synuclein (D37A6) XP® Rabbit mAb #4179 (lower) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

PhosphoPlus® α-Synuclein (Ser129) Antibody Duet: Image 4

Immunohistochemical analysis of paraffin-embedded mouse brain using α-Synuclein (D37A6) XP® Rabbit mAb.

PhosphoPlus® α-Synuclein (Ser129) Antibody Duet: Image 5

Confocal immunofluorescent analysis of mouse hippocampus using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (green). High-magnification showing punctate and sparse nuclear labeling (arrows). Specificity of nuclear staining is unclear (see Specificity/Sensitivity statement and supporting IF-F validation data). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

PhosphoPlus® α-Synuclein (Ser129) Antibody Duet: Image 6

Western blot analysis of extracts from mouse and rat brain using α-Synuclein (D37A6) XP® Rabbit mAb.

PhosphoPlus® α-Synuclein (Ser129) Antibody Duet: Image 7

Western blot analysis of extracts from mouse brain, untreated (-) or λ phosphatase-treated (+), using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (upper) or α-Synuclein (D37A6) XP® Rabbit mAb #4179 (lower).

To Purchase # 29087S
Product # Size Price
29087S
1 Kit $ 537

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-synuclein gene are linked to Parkinson's disease (1).

Various research studies have shown that phosphorylation of α-Synuclein at Ser129 is a highly toxic event that causes degeneration of dopaminergic neurons, which are associated with Parkinson's disease. This is proposed to occur through increased misfolding, aggregation, and accumulation of α-Synuclein phosphorylated at this site (2). GSK-3β is one of several kinases that has been reported to phosphorylate α-Synuclein at Ser129 (3).

  1. Goldberg, M.S. and Lansbury, P.T. (2000) Nat Cell Biol 2, E115-9.
  2. Oueslati, A. et al. (2010) Prog Brain Res 183, 115-45.
  3. Credle, J.J. et al. (2015) Cell Death Differ 22, 838-51.
  4. Yu, S. et al. (2007) Neuroscience 145, 539-55.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.