WB
H M R
Endogenous
65-80
Rabbit
#Q9UNQ0
9429
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Monkey, Xenopus, Bovine, Dog
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr180 of human ABCG2 protein, which lies within the intracellular region of ABCG2. Antibodies were purified by protein A and peptide affinity chromatography.
Background
ABCG2 (BCRP1/ABCP/MXR) is a member of the ATP-binding cassette transporter family that functions as ATP-dependent transporters for a wide variety of chemical compounds and are associated with drug-resistance in cancer cells (1-6). ABCG2 is a heavily glycosylated transmembrane protein with six transmembrane spanning regions consistent with it functioning as a half-transporter. The ABC family can exist as either full-length transporters or as half-transporters that form functional transporters through homo- or heterodimerization. High expression of ABCG2 was found in placenta as well as cell lines selected for resistance to a number of chemotherapeutic drugs, including mitoxantrone, doxorubicin, topotecan and flavopiridol. In rodents, the highest expression of ABCG2 was found in kidney (8). ABCG2 expression has also been observed in stem cell populations, particularly in hematopoietic and neuronal stem cells and is downregulated with differentiation (9-11).
- Doyle, L.A. and Ross, D.D. (2003) Oncogene 22, 7340-58.
- Allen, J.D. et al. (1999) Cancer Res 59, 4237-41.
- Doyle, L.A. et al. (1998) Proc Natl Acad Sci U S A 95, 15665-70.
- Allikmets, R. et al. (1998) Cancer Res 58, 5337-9.
- Miyake, K. et al. (1999) Cancer Res 59, 8-13.
- Robey, R.W. et al. (2001) Clin Cancer Res 7, 145-52.
- Zhou, S. et al. (2001) Nat Med 7, 1028-34.
- Honscha, W. et al. (2000) Hepatology 31, 1296-304.
- Scharenberg, C.W. et al. (2002) Blood 99, 507-12.
- Islam, M.O. et al. (2005) Neurosci Res 52, 75-82.
- Bunting, K.D. (2002) Stem Cells 20, 11-20.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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