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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb 11818 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence
H M R 280 Rabbit IgG
Acetyl-CoA Carboxylase (C83B10) Rabbit mAb 3676 40 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Hm 280 Rabbit IgG
Acetyl-CoA Carboxylase 1 Antibody 4190 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R 265 Rabbit 
Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb 8578 40 µl
Western Blotting Immunoprecipitation
H 280 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit provides an economical means of distinguishing between the two acetyl-CoA carboxylase isoforms, and between total acetyl-CoA carboxylase and phosphorylated acetyl-CoA carboxylase. The kit includes enough antibody to perform four western blot experiments per primary antibody.


Specificity / Sensitivity

Each antibody recognizes endogenous levels of their target protein. Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb recognizes endogenous levels of acetyl-CoA carboxylase protein only when phosphorylated at Ser79.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human acetyl-CoA carboxylase 1 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein, or a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase α1, or Val1416 of human acetyl-CoA carboxylase 2 protein.

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).


1.  Castle, J.C. et al. (2009) PLoS One 4, e4369.

2.  Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.

3.  Ha, J. et al. (1994) J Biol Chem 269, 22162-8.

4.  Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.

5.  Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

12704
Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit