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12704
Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit #12704

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Citations (1)
Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 1

Confocal immunofluorescent analysis of 293 cells (all nutrient-starved with Krebs-Ringer bicarbonate buffer for 4 hr), starved only (top left), serum-treated (10%, 30 min; top right), H2O2-treated (10 mM, 10 min; bottom left), or λ phosphatase-treated (2 hr; bottom right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 2

Flow cytometric analysis of SK-BR-3 cells (blue) and HT-29 cells (green) using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 3

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with either SignalSilence® ACC1 siRNA or SignalSilence® ACC2 siRNA, using Acetyl-CoA Carboxylase 1 Antibody and β-Actin (13E5) Rabbit mAb #4970.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 4

Western blot analysis of extracts from human adipocytes using Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 5

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 6

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 7

Confocal immunofluorescent analysis of NIH/3T3 cells labeled with Acetyl-CoA Carboxylase (C83B10) Rabbit mAb (red). Blue pseudocolor=Draq5™ (fluorescent DNA dye).

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 8

Western blot analysis of extracts from various cell types using Acetyl-CoA Carboxylase 1 Antibody.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 9

Immunohistochemical analysis of paraffin-embedded mouse liver using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 10

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb in the presence of control peptide (left) or Acetyl-CoA Carboxylase (C83B10) Blocking Peptide #1062 (right).

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 11

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 13

Immunohistochemical analysis of paraffin-embedded NCI-H2228 cell pellets, untreated (left) or phenformin-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 14

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 15

Western blot analysis of extracts from SH-SY5Y cells, untreated or treated with Oligomycin #9996 (0.5 μM, 30 min), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (upper) or Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (lower). The phospho-specificity of the antibody was verified by λ phosphatase treatment.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 16

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 17

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 18

Immunoprecipitation of Acetyl-CoA Carboxylase from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Acetyl-CoA Carboxylase (C83B10) Rabbit mAb. Western blot was performed using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit: Image 19

Western blot analysis of cell extracts from various cell lines, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

To Purchase # 12704T
Product # Size Price
12704T
1 Kit  (4 x 20 µl) $ 327

Product Description

The Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit provides an economical means of distinguishing between the two acetyl-CoA carboxylase isoforms, and between total acetyl-CoA carboxylase and phosphorylated acetyl-CoA carboxylase. The kit includes enough antibody to perform two western blot experiments per primary antibody.

Specificity / Sensitivity

Each antibody recognizes endogenous levels of their target protein. Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb recognizes endogenous levels of acetyl-CoA carboxylase protein only when phosphorylated at Ser79.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human acetyl-CoA carboxylase 1 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein, or a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase α1, or Val1416 of human acetyl-CoA carboxylase 2 protein.

Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).

  1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
  2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
  3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
  4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
  5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.