Render Target: STATIC
Render Timestamp: 2025-03-20T10:41:24.353Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:20:32.857
Product last modified at: 2025-03-06T19:00:09.019Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

AHCY/SAHH (F2O1K) Rabbit mAb #46619

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 45
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    AHCY/SAHH (F2O1K) Rabbit mAb recognizes endogenous levels of total AHCY/SAHH protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AHCY/SAHH protein.

    Background

    Adenosylhomocysteinase (AHCY), also known as S-adenosyl-L-homocysteine hydrolase (SAHH), catalyzes the hydrolysis of SAH to generate homocysteine and adenosine (1,2). SAH is a by-product in the transmethylation reactions catalyzed by S-adenosylmethionine (SAM)-dependent methyltransferases. These methylation reactions are critical to various biological processes. SAH is also a feedback inhibitor of SAM-dependent methyltransferases. By catalyzing the above reaction, AHCY/SAHH plays a crucial role in relieving the inhibition (3). Moreover, studies show that hydrogen sulfide (H2S) persulfidates Cys195 on AHCY/SAHH in human non-small cell lung cancer (NSCLC) cells and therefore decreases its enzymatic activity, resulting in reduced homocysteine levels. This, in turn, lowers cysteine and glutathione levels in the cell when cystine is depleted, making NSCLC cells more susceptible to ferroptosis (2,4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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