Revision 8
#3633
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, W-S, IP, IHC-P, FC-FP
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
220 (ALK), 80 (NPM-ALK), 117 (EML4-ALK v1), 86 (EML4-ALK v3)
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q9UM73
Entrez-Gene Id:
238
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:2000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:800 |
Storage
Specificity/Sensitivity
Source / Purification
Background
A distinct ALK oncogenic fusion protein involving ALK and echinoderm microtubule-associated protein like 4 (EML4) has been described in the research literature from a non-small cell lung cancer (NSCLC) cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6-8).
Investigators have identified ALK translocations with other fusion partners, such as TRK-fused gene (TFG) and KIF5B, which have also been associated with NSCLC (6,7). In particular, the EML4-ALK fusion protein has been found in 3-7% of NSCLC samples (6-14).
Background References
- Stoica, G.E. et al. (2001) J Biol Chem 276, 16772-9.
- Iwahara, T. et al. (1997) Oncogene 14, 439-49.
- Morris, S.W. et al. (1997) Oncogene 14, 2175-88.
- Morris, S.W. et al. (1994) Science 263, 1281-4.
- Bai, R.Y. et al. (1998) Mol Cell Biol 18, 6951-61.
- Rikova, K. et al. (2007) Cell 131, 1190-203.
- Takeuchi, K. et al. (2008) Clin Cancer Res 14, 6618-24.
- Soda, M. et al. (2007) Nature 448, 561-6.
- Takeuchi, K. et al. (2009) Clin Cancer Res 15, 3143-9.
- Palmer, R.H. et al. (2009) Biochem J 420, 345-61.
- Horn, L. and Pao, W. (2009) J Clin Oncol 27, 4232-5.
- Rodig, S.J. et al. (2009) Clin Cancer Res 15, 5216-23.
- Mino-Kenudson, M. et al. (2010) Clin Cancer Res 16, 1561-71.
- Kwak, E.L. et al. (2010) N Engl J Med 363, 1693-703.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting W-S: Simple Western™ IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: Human
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
D5F3 is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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Revision 8
Revision 8
