WB, IP
H Mk
Endogenous
44
Rabbit
#Q13686
8846
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ALKBH1 protein. Antibodies are purified by peptide affinity chromatography.
Background
AlkB is an oxidative dealkylating DNA repair enzyme first characterized in E.coli (1-5). Nine AlkB homologs exist in mammals, with the first eight designated as ALKBH1-ALKBH8, and the ninth as FTO (fat mass and obesity-associated protein) (6). ALKBH1, which features the highest sequence identity to E.coli AlkB, is an Fe(II) and 2-oxoglutarate-dependent dioxygenase that acts upon nucleic acids such as DNA and tRNA and carries out a wide range of enzymatic functions (6,7). Similar to other AlkB proteins, ALKBH1 is able to repair alkylated single-stranded DNA and RNA containing 3-methylcytosine (m3C), albeit with weak activity (8). Perhaps more importantly, it has also been shown to catalyze the demethylation of N1-methyladenosine on tRNAs to regulate translation (9). ALKBH1 functions in the mitochondria as well, recognizing and oxidizing 5-methylcytosine (m5C) on mitochondrial tRNAMet to generate 5-formylcytosine, consequently enhancing mitochondrial translation (10). Interestingly, ALKBH1 has also been shown to possess apurinic/apyrimidinic (AP) lyase activity, cleaving both single-stranded and double-stranded DNA at abasic sites, with greatest affinity toward double-stranded DNA with two abasic sites (11). Lastly, ALKBH1 has been reported to possess N(6)-methyladenine (6mA) demethylase activity, suggesting a role in epigenetic regulation (12,13). However, an additional study was unable to show definitive ALKBH1 6mA demethylase activity using both biochemistry assays and knockout mice, so this enzymatic function remains controversial (9).
- Samson, L. and Cairns, J. (1977) Nature 267, 281-3.
- Chen, B.J. et al. (1994) J Bacteriol 176, 6255-61.
- Aravind, L. and Koonin, E.V. (2001) Genome Biol 2, RESEARCH0007.
- Trewick, S.C. et al. (2002) Nature 419, 174-8.
- Falnes, P.Ø. et al. (2002) Nature 419, 178-82.
- Fedeles, B.I. et al. (2015) J Biol Chem 290, 20734-42.
- Müller, T.A. et al. (2018) Biochem Biophys Res Commun 495, 98-103.
- Westbye, M.P. et al. (2008) J Biol Chem 283, 25046-56.
- Liu, F. et al. (2016) Cell 167, 816-828.e16.
- Haag, S. et al. (2016) EMBO J 35, 2104-19.
- Müller, T.A. et al. (2010) DNA Repair (Amst) 9, 58-65.
- Xiao, C.L. et al. (2018) Mol Cell 71, 306-318.e7.
- Wu, T.P. et al. (2016) Nature 532, 329-33.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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