WB
H
Endogenous
70
Mouse IgG2b
#P05187
250
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human ALPP protein.
Background
Mammalian alkaline phosphatases (APs) are highly conserved zinc-containing allosteric enzymes that are able to hydrolyze and transphosphorylate a wide range of compounds (1). There are four known human alkaline phosphatase isozymes: TNAP (tissue-nonspecific; bone/liver/kidney), ALPP (placental), ALPP2 (germ cell), and ALPI (intestinal) (2). Placental alkaline phosphatase (ALPP) is bound to the plasma membrane via a glycosyl-phosphatidylinositol (GPI) anchor (3). It is expressed primarily in the placenta (4) and may be involved in transplacental IgG transport (5). ALPP has been found to be overexpressed on the surface of several different types of solid tumor cells (6) and elevated serum concentrations of ALPP and ALPP-like enzymes has been found to be associated with ovarian, cervical, and testicular cancer (7).
- Hoylaerts, M.F. et al. (1997) J Biol Chem 272, 22781-7.
- Millán, J.L. (2006) Purinergic Signal 2, 335-41.
- Low, M.G. and Saltiel, A.R. (1988) Science 239, 268-75.
- Beckman, G. et al. Hum Hered 45, 1-5.
- Fishman, L. et al. (1976) Cancer Res 36, 2268-73.
- Ind, T. et al. (1997) Br J Obstet Gynaecol 104, 1024-9.
- Koshida, K. et al. (1996) Br J Urol 77, 138-42.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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