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9784
Alzheimer's Disease Antibody Sampler Kit

Alzheimer's Disease Antibody Sampler Kit #9784

Western Blotting Image 1

Western blot analysis of human Aβ-42, Aβ-40, Aβ-39, Aβ-38, and Aβ-37 peptides (5 ng) using β-Amyloid (D54D2) XP® Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of extracts from mouse brain, HeLa cells and rat brain, using Neurofilament-L (C28E10) Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of extracts from mouse and rat brain, using Tau (Tau46) Mouse mAb.

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Western Blotting Image 4

Western blot analysis of extracts from HeLa cells, untransfected and BACE transfected, and rat brain extracts using BACE (D10E5) Rabbit mAb.

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Western Blotting Image 5

Western blot analysis of extracts from HeLa and SK-N-MC cells, using APP/beta-Amyloid (NAB228) Mouse mAb.

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Western Blotting Image 6

Western blot analysis of extracts from human cerebellum and HeLa cells, using α-Synuclein (Syn204) Mouse mAb.

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Western Blotting Image 7

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® GSK-3α/β siRNA #6301, using GSK-3α/β (D75D3) XP® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

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Western Blotting Image 8

Western blot analysis of extracts from COS-7 cells, λ-phosphatase or PDGF-treated, using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb (upper) or GSK-3α Antibody #9338 (lower).

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Western Blotting Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Western Blotting Image 10

Western blot analysis of the indicated amounts of human Aβ-42 (left) and Aβ-40 (right) peptides using β-Amyloid (D54D2) XP® Rabbit mAb.

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded mouse brain using Neurofilament-L (C28E10) Rabbit mAb.

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded Alzheimer's brain, using Tau (Tau46) Mouse mAb.

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IF-IC Image 13

Confocal immunfluorescent analysis of H4 expressing BACE (left), or H4 wild-type, (right) cells using BACE (D10E5) Rabbit mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (H4 wild type and H4 cells expressing BACE were kindly provided by Dr. Giuseppina Tesco, Tufts University, Boston, MA).

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IHC-P (paraffin) Image 14

Immunohistochemical analysis of paraffin-embedded Alzheimer's brain, using APP/beta-Amyloid (NAB228) Mouse mAb.

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IHC-P (paraffin) Image 15

Immunohistochemical analysis of paraffin-embedded human brain, using α-Synuclein (Syn204) Mouse mAb.

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Western Blotting Image 16

Western blot analysis of extracts from GSK-3β (-/-) (lanes 1,2), GSK-3α (-/-) (lanes 3,4) and wild type (lanes 5,6) mouse embryonic fibroblast cells (MEF), untreated or insulin treated, using GSK-3α/β (D75D3) XP® Rabbit mAb. (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

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IHC-P (paraffin) Image 17

Immunohistochemical analysis of paraffin-embedded human breast carcinoma , untreated (left) or lambda phosphatase treated (right), using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb.

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Western Blotting Image 18

Western blot analysis of human Aβ-42 peptide (1 ng) and human cerebrospinal fluid (CSF) of an AD patient using β-Amyloid (D54D2) XP® Rabbit mAb.

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IHC-P (paraffin) Image 19

Immunohistochemical analysis of paraffin-embedded human brain using Neurofilament-L (C28E10) Rabbit mAb in the presence of control peptide (left) or Neurofilament-L blocking peptide #1005 (right).

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IF-F Image 20

Confocal immunofluorescent analysis of mouse dentate gyrus stained with Tau (Tau46) Mouse mAb (red) and CREB (86B10) Mouse mAb #9104 (blue).

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IF-F Image 21

Confocal immunofluorescent analysis of mouse hippocampus using BACE (D10E5) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IF-P Image 22

Confocal immunofluorescent analysis of paraffin-embedded human Alzheimer's brain using APP/β-Amyloid (NAB228) Mouse mAb (green) and Phospho-p44/42 MAPK (Thr202/Tyr204) (197G2) Rabbit mAb #4377 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

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IHC-P (paraffin) Image 23

Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right), using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb.

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IF-P Image 24

Confocal immunofluorescent analysis of paraffin-embedded human Alzheimer's brain using β-Amyloid (D54D2) XP® Rabbit mAb (green) and Tau (Tau46) Mouse mAb #4019 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IF-F Image 25

Confocal immunofluorescent analysis of normal rat cerebellum using Neurofilament-L (C28E10) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IF-P Image 26

Confocal immunofluorescent analysis of human Alzheimer brain (A) labeled with Tau (Tau46) Mouse mAb (green) and APP Antibody #2452 (red) compared to normal human brain (B).

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IHC-P (paraffin) Image 27

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb in the presence of control peptide (left) or Phospho-GSK-3α (Ser21) (36E9) Blocking Peptide #1027 (right).

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IHC-P (paraffin) Image 28

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
β-Amyloid (D54D2) XP® Rabbit mAb 8243 20 µl
  • WB
  • IP
  • IF
H 5 Rabbit IgG
Neurofilament-L (C28E10) Rabbit mAb 2837 20 µl
  • WB
  • IHC
  • IF
H M R 70 Rabbit IgG
Tau (Tau46) Mouse mAb 4019 20 µl
  • WB
  • IHC
  • IF
H M R 50 to 80 Mouse IgG1
BACE (D10E5) Rabbit mAb 5606 20 µl
  • WB
  • IP
  • IF
H M R 70 Rabbit IgG
APP/β-Amyloid (NAB228) Mouse mAb 2450 20 µl
  • WB
  • IHC
  • IF
H Mk B 100 to 140 Mouse IgG2a
α-Synuclein (Syn204) Mouse mAb 2647 20 µl
  • WB
  • IHC
H 18 Mouse IgG2a
GSK-3α/β (D75D3) Rabbit mAb 5676 20 µl
  • WB
  • IP
H M R Hm Mk 51, 46 Rabbit IgG
Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb 9316 20 µl
  • WB
  • IHC
H M R Mk 51 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 

The Alzheimer's Disease Antibody Sampler Kit provides an economical means of evaluating Alzheimer's Disease-related signaling. The kit contains enough primary and secondary antibodies to perform two western blot experiments per primary antibody.

β-Amyloid (D54D2) XP® Rabbit mAb recognizes endogenous levels of total β-amyloid peptide (Aβ). The antibody detects several isoforms of Aβ, such as Aβ-37, Aβ-38, Aβ-39, Aβ-40, and Aβ-42. APP/β-Amyloid (NAB228) Mouse mAb detects endogenous levels of APP/β-Amyloid protein. Although this antibody recognizes both the phospho and non-phospho forms of the protein, it has been shown to prefer the phosphorylated form in some systems. BACE (D10E5) Rabbit mAb detects endogenous levels of total BACE protein. Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb detects endogenous levels of GSK-3α protein when phosphorylated at Ser21, and does not detect GSK-3β when phosphorylated at Ser9. GSK-3α/β (D75D3) XP® Rabbit mAb detects endogenous levels of total GSK-3α and GSK-3β protein. The antigen is 100% conserved between GSK-3α and GSK-3β in humans, monkeys, mice, and rats. Neurofilament-L (C28E10) Rabbit mAb detects endogenous levels of total Neurofilament-L protein. α-Synuclein (Syn204) Mouse mAb detects endogenous levels of total synuclein protein. This antibody detects recombinant α but not β-synuclein (Giasson, B.I. et al., 2000). Tau (Tau46) Mouse mAb detects endogenous levels of total tau protein, and also cross-reacts with MAP2 at 280 kDa. This antibody is predicted to detect all six isoforms of tau based on the amino acid sequence.

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ß-amyloid peptide (Aß), surrounding Glu450 of human Neurofilament-L protein, native bovine tau protein (carboxy terminus), a synthetic peptide corresponding to residues surrounding Asp490 of human BACE protein, human recombinant α-synuclein protein (amino terminus), ß-amyloid protein (amino terminus) (Lee et al., 2003), a synthetic peptide surrounding Gln269 of human GSK-3α protein, or a synthetic phosphopeptide corresponding to residues surrounding Ser21 of human GSK-3α protein

Alzheimer's Disease (AD) is one of the most common neurodegenerative diseases worldwide. Clinically, it is characterized by the presence of extracellular amyloid plaques and intracellular neurofibrillary tangles, which results in neuronal dysfunction and cell death. Central to this disease is the differential processing of the integral transmembrane glycoprotein Amyloid β (A4) precursor protein (APP) that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). β-secretase (BACE) is an aspartic acid proteinase that catalyses the initial step in APP processing by cleaving and releasing a soluble, extracellular APP-β (sAPPβ) ectodomain and generating a membrane-bound, carboxy-terminal fragment consisting of 99 amino acids (CTF99). Additional processing of CTF99 by γ-secretase generates the amyloid β-peptide (Aβ) that forms aggregates in the brains of AD patients. BACE is an attractive target for inhibitors in AD therapy since it catalyses the first and rate limiting step in amyloidogenic APP processing (2). Pro-BACE-1 is synthesized in the ER before it is transported to the trans-Golgi network to undergo maturation (3). The extracellular deposition and accumulation of the released Aβ fragments and an α-synuclein fragment known as the non- Aβ fragment, form the main components of amyloid plaques in AD. GSK-3α regulates the production of Aβ peptides. Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates Aβ production by specifically inhibiting the cleavage of APP by γ-secretase, thereby blocking accumulation of Aβ peptides in the brains of mice that overproduce APP (4). AD is also characterized by the presence of neurofibrillary tangles. These tangles are the result of hyperphosphorylation and oligomerization of the microtubule associated protein Tau and lead to apoptosis of the neuron. In particular, phosphorylation of Tau Ser396 by GSK-3 or CDK5 destabilizes microtubules in AD (5,6). Additionally, neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM) and heavy (NFH) subunits (7). Accumulation of neurofilaments are found in many human neurological disorders including AD (7).

  1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
  2. Hunt, C.E. and Turner, A.J. (2009) FEBS J 276, 1845-59.
  3. Walter, J. et al. (2001) J Biol Chem 276, 14634-41.
  4. Phiel, C.J. et al. (2003) Nature 423, 435-9.
  5. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  6. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
  7. Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-55.
Entrez-Gene Id
351 , 23621 , 2931 , 2932 , 4747 , 6622 , 4137
Swiss-Prot Acc.
P05067 , P56817 , P49840 , P49841 , P07196 , P37840 , P10636-8
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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