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PhosphoSitePlus® Resource

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb 2478 20 µl
Western Blotting Immunohistochemistry Immunofluorescence
H M 230 Rabbit IgG
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb 4060 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Hm Mk Dm Z B 60 Rabbit IgG
Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb 6943 20 µl
Western Blotting Immunoprecipitation
H M R Mk 60 Rabbit IgG
Phospho-FAK (Tyr397) (D20B1) Rabbit mAb 8556 20 µl
Western Blotting Immunoprecipitation
H 125 Rabbit IgG
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb 4511 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk Mi Pg Sc 43 Rabbit IgG
Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb 8713 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M Mk 150 Rabbit IgG
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb 4370 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Hm Mk Mi Dm Z B Dg Pg Sc 44, 42 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The Angiogenesis Antibody Sampler Kit provides an economical means to investigate the angiogenic pathway downstream of VEGFR2. The kit contains enough primary antibody to perform two western blots per primary antibody.


Specificity / Sensitivity

Each antibody in the Angiogenesis Antibody Sampler Kit recognizes the phosphorylated form of its specific target. Phospho-FAK (Tyr397) (D20B1) Rabbit mAb may cross-react with overexpressed tyrosine phosphorylated proteins such as EGFR. Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb may cross-react with other Src family members or overexpressed phosphorylated RTKs. Phospho-VEGFR2 (Tyr1175) (19A10) Rabbit mAb may cross-react with VEGFR1.


Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser473 of human Akt protein, Tyr397 of human FAK protein, Thr180/Tyr182 of human p38 MAPK protein, Thr202/Tyr204 of human p44 MAPK protein, Ser1248 of human PLCγ1 protein, Tyr416 of human Src protein, and Tyr1175 of human VEGFR2 protein.

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Signaling from VEGFR2 is necessary for angiogenesis in vivo (2-4). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (5). Phosphorylation of VEGFR2 at Tyr1212 provides a docking site for GRB2 binding and phosphorylation at Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ (1,5,6). Activation of VEGFR2 during angiogenesis leads to signaling through multiple downstream kinase pathways including Akt, Src, FAK, p38, and Erk1/2 (2,7).


1.  Meyer, M. et al. (1999) EMBO J 18, 363-74.

2.  Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.

3.  Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.

4.  Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

5.  Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.

6.  Takahashi, T. et al. (2001) EMBO J 20, 2768-78.

7.  Le Boeuf F et al. (2004) J Biol Chem 279, 39175–85


Entrez-Gene Id 207 , 208 , 10000 , 5595 , 5594 , 5747 , 2534 , 3055 , 3932 , 4067 , 1432 , 5600 , 5603 , 6300 , 5335 , 6714 , 3791 , 7525


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.
U.S. Patent No. 5,675,063.

8696
Angiogenesis Antibody Sampler Kit