WB
H
Endogenous
68, 70
Rabbit
#O15123
285
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp200 of human angiopoietin-2 protein. Antibodies are purified by peptide affinity chromatography.
Background
Angiopoietins are a family of Tie receptor ligands. There are four angiopoietins discovered so far: angiopoietins 1, 2, 3 and 4 (Ang1, 2, 3, and 4) (1-3). Ang1 binds to the Tie-2 receptor and leads to its autophosphorylation and subsequent activation of downstream signaling pathways. It plays an important role in blood vessel formation, maturation and subsequent stabilization (1,4,5). Ang2 is an endothelium-specific growth factor that functions as an antagonist to Ang1, promotes vascular associated proinflammatory function, destabilizes quiescent endothelium, leads to vascular leakage and vascular destabilization and remodeling (2,6,7). Ang2 is selectively expressed in many tumor tissues where, combined with other growth factors such as VEGF, it can promote vascular remodeling, angiogenesis and inflammation (7-9).
- Davis, S. et al. (1996) Cell 87, 1161-9.
- Maisonpierre, P.C. et al. (1997) Science 277, 55-60.
- Valenzuela, D.M. et al. (1999) Proc Natl Acad Sci USA 96, 1904-9.
- Suri, C. et al. (1996) Cell 87, 1171-80.
- Thurston, G. et al. (1999) Science 286, 2511-4.
- Gale, N.W. et al. (2002) Dev Cell 3, 411-23.
- Fiedler, U. et al. (2006) Nat Med 12, 235-9.
- Tait, C.R. and Jones, P.F. (2004) J Pathol 204, 1-10.
- Tsutsui, S. et al. (2006) Breast Cancer Res Treat 98, 261-6.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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