WB
H M R
Endogenous
160
Rabbit IgG
#Q14678
23189
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
ANKRD15 (E9I4I) Rabbit mAb recognizes endogenous levels of total ANKRD15 protein. This antibody detects a nonspecific band at 48 kDa by western blot.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ANKRD15 protein.
Background
ANKRD15 (ankyrin repeat domain-containing protein 15, KANK1) belongs to the Kank family of proteins, which contain multiple ankyrin repeat domains and are essential for crosstalk between actin and microtubules (1). ANKRD15 was initially identified as a potential tumor suppressor for renal cell carcinoma and has since been linked to gastric cancer, colorectal cancer, and oral squamous carcinoma (1,2). The protein regulates actin polymerization and cell motility and has two major alternatively spliced transcripts with different promoters and several other possible transcripts (3). Loss-of-function mutations have since been linked to congenital cerebral palsy and other neurodevelopmental disorders (3,4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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