WB, IP
H M
Endogenous
80
Rabbit
#Q9H6X2
84168
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg557 of human ANTXR1/TEM8 protein. Antibodies are purified by peptide affinity chromatography.
Background
ANTXR1 (Anthrax toxin receptor 1, TEM8, tumor endothelial marker 8) is a protein biomarker induced in tumor-associated endothelia and acts as a cell surface receptor for the anthrax toxin and the oncolytic Seneca Valley Virus (1-3). The protein is a type I transmembrane glycoprotein that is expressed on the epithelial cell surface of skin, lung, and intestine tissues as a docking site for toxin/virus binding and endocytosis, and in tumor endothelial cells for tumor-associated vascular development (4). ANTXR1 interacts with type I and type VI collagens in the extracellular matrix, and with actin cytoskeleton in the cytoplasm to promote tumor-associated endothelial cell adhesion, migration, and spreading during angiogenesis (4-6). Aberrant high expression of ANTXR1 has been found in various cancer types including metastatic breast cancer cells, pancreatic cancer stem cells, etc. (7-9); there the protein promotes tumor proliferation, growth, and invasion. Because of its specific high expression in tumors and to their surrounding vasculature, ANTXR1 has been proposed as a target for CAR T cell therapy for cancer treatment (10,11).
- St Croix, B. et al. (2000) Science 289, 1197-202.
- Bradley, K.A. et al. (2001) Nature 414, 225-9.
- Evans, D.J. et al. (2018) Front Oncol 8, 506.
- Cryan, L.M. and Rogers, M.S. (2011) Front Biosci (Landmark Ed) 16, 1574-88.
- Werner, E. et al. (2006) J Biol Chem 281, 23227-36.
- Nanda, A. et al. (2004) Cancer Res 64, 817-20.
- Opoku-Darko, M. et al. (2011) Cancer Invest 29, 676-82.
- Alcalá, S. et al. (2019) Stem Cells Int 2019, 1378639.
- Sergeeva, O.A. and van der Goot, F.G. (2019) F1000Res 8, F1000 Faculty Rev-1415.
- Byrd, T.T. et al. (2018) Cancer Res 78, 489-500.
- Sotoudeh, M. et al. (2019) J Cell Biochem 120, 5010-5017.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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