WB, IP
H M R
Endogenous
100
Rabbit IgG
#O43747
164
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AP1G1 protein.
Background
The adaptor protein complex 1 (AP-1) is a heterotetrameric protein complex composed of two large adaptins (AP1G1 and AP1B1), a medium adaptin (AP1M1 or AP1M2), and one of several small adaptins (AP3S1, AP1S2, or AP1S3). AP-1 complexes containing AP1M1 are ubiquitously expressed, while complexes containing AP1M2 display a more restricted tissue expression pattern. Research studies have demonstrated that the AP-1 complex is localized to the trans-Golgi network and recycling endosomes where it regulates the bidirectional trafficking of cargo proteins between these subcellular compartments as well as biogenesis of secretory vesicles (1-3). The AP-1 complex recognizes dileucine-based sorting motifs within the cytoplasmic tail of cargo proteins, in part, via the AP1G1 subunit (4). In mice, biallelic knockout of Ap1g1 is embryonic lethal due to disruption of AP-1 complex stability, highlighting the importance of this subunit to the structure and function of AP-1 (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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