Revision 1

#26081

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

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For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
80

Source/Isotype:
Rabbit

UniProt ID:
#Q9Y6B7

Entrez-Gene Id:
10717

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

AP4B1 Antibody recognizes endogenous levels of total human AP4B1 protein. Weak reactivity has been observed in rodent.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile708 of human AP4B1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

AP (adaptor protein) complexes regulate intracellular membrane trafficking along endocytic and secretory pathways (1). There are five AP complexes (AP1-5) that exist as heterotetrameric complexes functioning at distinct intracellular compartments. The AP4 complex, which includes AP4E1, AP4B1, AP4M1, and AP4S1, functions at the trans-Golgi network (TGN) (2-4). AP-4 associates with Atg9A which is critical in autophagosome biogenesis (5). Deficiency in AP-4 led to Atg9A retention within the trans-Golgi network with defective axonal autophagosome generation (5). AP-4 deficiency has been linked to a group of neurodegenerative diseases known as hereditary spastic paraplegia. Mutation in the AP4B1 gene was identified as a cause for the neurological disorder hereditary spastic paraplegia type 47 (SPG47) (6,7).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.