WB
H
Endogenous
80
Rabbit
#Q9Y6B7
10717
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile708 of human AP4B1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
AP (adaptor protein) complexes regulate intracellular membrane trafficking along endocytic and secretory pathways (1). There are five AP complexes (AP1-5) that exist as heterotetrameric complexes functioning at distinct intracellular compartments. The AP4 complex, which includes AP4E1, AP4B1, AP4M1, and AP4S1, functions at the trans-Golgi network (TGN) (2-4). AP-4 associates with Atg9A which is critical in autophagosome biogenesis (5). Deficiency in AP-4 led to Atg9A retention within the trans-Golgi network with defective axonal autophagosome generation (5). AP-4 deficiency has been linked to a group of neurodegenerative diseases known as hereditary spastic paraplegia. Mutation in the AP4B1 gene was identified as a cause for the neurological disorder hereditary spastic paraplegia type 47 (SPG47) (6,7).
- Park, S.Y. and Guo, X. (2014) Biosci Rep 34, e00123. doi: 10.1042/BSR20140069.
- Boehm, M. et al. (2001) EMBO J 20, 6265-76.
- Hirst, J. et al. (1999) Mol Biol Cell 10, 2787-802.
- Dell'Angelica, E.C. et al. (1999) J Biol Chem 274, 7278-85.
- Ivankovic, D. et al. (2020) Autophagy 16, 391-407.
- Bauer, P. et al. (2012) Neurogenetics 13, 73-6.
- Ebrahimi-Fakhari, D. et al. (2018) Am J Med Genet A 176, 311-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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