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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
21

Source/Isotype:
Rabbit

UniProt ID:
#Q9NVJ2

Entrez-Gene Id:
55207

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

ARL8B Antibody recognizes endogenous levels of total ARL8B protein. This antibody does not cross-react with ARL8A.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala137 of human ARL8B protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The small GTPases of the Arf and Rab families have critical roles in cytoskeletal organization and membrane/vesicular transport (1,2). The related ADP-ribosylation factor-like (ARL) family member ARL8, including two paralogs ARL8A and ARL8B, has emerged as an important regulator of lysosomal transport to the cell periphery (3,4). Lysosome positioning is a critical determinant of a number of physiological processes including plasma membrane repair, cell migration, antigen presentation, and response to nutrient availability. Membrane association of ARL8B is regulated by the BLOC-one related complex (BORC), a multi-subunit complex under the control of the mTOR pathway (5,6). Effectors of GTP-bound ARL8B include PLEKHM2/SKIP and the Vps41 subunit of the HOPS complex (7). Studies have found that ARL8B may be a critical factor for NK-mediated cytotoxicity by driving polarization of lytic granules toward the immune synapse (8). In addition, ARL8B has been shown to act as a negative regulator of autophagy (9). Knockdown of ARL8B resulted in increased fusion of autophagosomes with lysosomes. Furthermore, ARL8B depleted cells had reduced levels of pathogenic substrates associated with neurodegenerative diseases.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.