WB, IP, IHC-P
H
Endogenous
50
Rabbit IgG
#P11511
1588
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:4200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human aromatase protein.
Background
Aromatase is a member of the cytochrome P450 superfamily of enzymes, which are monooxygenases that catalyze reactions involved in drug metabolism and cholesterol and steroid synthesis (1,2). Aromatase is responsible for the conversion of testosterone into 17-β estradiol (2). Aromatase is mainly expressed in the brain (3), ovaries (4), and placenta (5). Aromatase plays an important role in development of the central nervous system during ontogenesis (6,7), gonadal development, and sex differentiation (8,9). Research studies have suggested that inhibition of aromatase may be an effective therapeutic strategy for postmenopausal breast cancers that are estrogen receptor positive (6,10). Mutations in the corresponding aromatase gene are associated with cases of aromatase excess syndrome (AEXS) and aromatase deficiency (AROD) disorders (11-14).
- Harada, N. (1988) Biochem Biophys Res Commun 156, 725-32.
- Chen, S.A. et al. (1988) DNA 7, 27-38.
- Hinshelwood, M.M. et al. (2000) Endocrinology 141, 2050-3.
- Pezzi, V. et al. (2003) J Steroid Biochem Mol Biol 87, 181-9.
- Peruffo, A. et al. (2011) Gen Comp Endocrinol 172, 211-7.
- Montelli, S. et al. (2012) Brain Res 1475, 11-8.
- Kuntz, S. et al. (2003) Cytogenet Genome Res 101, 283-8.
- Ramsey, M. et al. (2007) Differentiation 75, 978-91.
- Hu, Q. et al. (2012) J Med Chem 55, 7080-9.
- Gilani, R.A. et al. (2012) Breast Cancer Res Treat 135, 681-92.
- Ito, Y. et al. (1993) Proc Natl Acad Sci U S A 90, 11673-7.
- Morishima, A. et al. (1995) J Clin Endocrinol Metab 80, 3689-98.
- Carani, C. et al. (1997) N Engl J Med 337, 91-5.
- Fukami, M. et al. (2012) Int J Endocrinol 2012, 584807.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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