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Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB

REACTIVITY:

H Mk

SENSITIVITY:

Endogenous

MW (kDa):

210

Source/Isotype:

Rabbit IgG

UniProt ID:

#Q76L83

Entrez-Gene Id:

55252

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

ASXL2 (E6Z3X) Rabbit mAb recognizes endogenous levels of total ASXL2 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala640 of human ASXL2 protein.

Background

Additional sex combs-like protein 2 (ASXL2) is a polycomb-associated protein that interacts with polycomb repressive complex 2 (PRC2), which contains the histone methyltransferase EZH2 and functions to mono-, di-, and tri-methylate histone H3 on lysine 27. These histone marks are associated with transcriptional repression (1). In addition, ASXL2 interacts with BRCA1-associated protein 1 (BAP1), the catalytic subunit of the polycomb repressive deubiquitinase complex (PR-DUB), which functions to de-ubiquitinate histone H2A at lysine 119 and activate transcription (2). ASXL2 functions as a transcriptional regulator of adipogenesis, acting to induce peroxisome proliferator-activated receptor gamma (PPARG) adipocyte differentiation, whereas its paralog ASXL1 functions to inhibit PPARG activity (3). ASXL2 is critical for animal viability and growth, as ASXL2 knockout mice experience partial embryonic lethality, premature death, axial skeletal abnormalities, and reduced bone mineral density (4,5). ASXL2 also plays a role in cancer, where ASXL2 truncation mutations are found in pancreatic, breast, and castration-resistant prostate cancers, albeit infrequently (6-9). ASXL2 is also frequently mutated in acute myeloid leukemia patients with t(8;21)/RUNX1-RUNX1T1 chromosomal translocations, and these mutations correlate with a 36% incidence of relapse (10).

  1. Abdel-Wahab, O. et al. (2012) Cancer Cell 22, 180-93.
  2. Daou, S. et al. (2015) J Biol Chem 290, 28643-63.
  3. Park, U.H. et al. (2011) J Biol Chem 286, 1354-63.
  4. Baskind, H.A. et al. (2009) PLoS One 4, e4750.
  5. Farber, C.R. et al. (2011) PLoS Genet 7, e1002038.
  6. Katoh, M. (2013) Br J Cancer 109, 299-306.
  7. Jones, S. et al. (2008) Science 321, 1801-6.
  8. Stephens, P.J. et al. (2012) Nature 486, 400-4.
  9. Grasso, C.S. et al. (2012) Nature 487, 239-43.
  10. Micol, J.B. et al. (2014) Blood 124, 1445-9.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.