WB, IP, IHC-P, IF-IC
H M R Mk
Endogenous
71
Rabbit IgG
#P11940
26986
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:25 |
| Immunohistochemistry (Paraffin) | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Chicken, Horse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PABP1 in which Arg455 and Arg460 are methylated.
Background
Poly(A)-binding protein 1 (PABP1) associates with the 3' poly(A) tail of mRNA and also eIF4F (1,2). eIF4F is a complex whose functions include the recognition of the mRNA 5' cap structure (eIF4E), delivery of an RNA helicase to the 5' region (eIF4A), bridging of the mRNA and the ribosome (eIF4G), and circularization of the mRNA via interaction between eIF4G and the poly(A) binding protein (PABP). PABP1 has been shown to have multiple functions including translation initiation, mRNA stabilization, and mRNA turnover (3,4). Phosphorylation of PABP has been shown to enhance RNA binding in eukaryotes, and PABP1 has been shown to shuttle between the nucleus and cytoplasm (5,6). PABP1 is methylated on Arg455 and Arg460 by the CARM1 protein methyltransferase (7,8); however, the function of this methylation has yet to be determined.
- Sachs, A. B. et al. (1986) Cell 45, 827-835.
- Piron, M. et al. (1998) EMBO J. 17, 5811-5821.
- Caponigro, G. and Parker, R. (1995) Genes Dev. 9, 2421-2432.
- Sachs, A.B. and Davis, R.W. (1989) Cell 58, 857-867.
- Le, H. et al. (2000) J. Biol. Chem. 275, 17452-17462.
- Afonina, E. et al. (1998) J. Biol. Chem. 273, 13015-13021.
- Lee, J. and Bedford, M.T. (2002) EMBO Rep 3, 268-73.
- Yadav, N. et al. (2003) Proc Natl Acad Sci U S A 100, 6464-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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