WB, IP, IF-IC, FC-FP, ChIP
H M
Endogenous
23, 24
Rabbit IgG
#P18847
467
Product Information
Product Usage Information
and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
| Chromatin IP | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human ATF-3 protein.
Background
Activating transcription factor 3 (ATF-3) is a basic leucine zipper-type transcription factor belonging to the ATF/cAMP responsive element binding protein family. ATF-3 can form homodimers or heterodimers with other family members and depending on the cell types and promoter context, it can suppress or activate the transcription of its target genes (1,2). Expression of ATF-3 is induced by a variety of factors including cytokines, genotoxic agents, and physiological stress. For example, both metformin and high-density lipoprotein (HDL) can induce ATF-3 expression in macrophages (1,3-5). Research studies also indicate that ATF-3 can function as an oncogene or a tumor suppressor depending on the context (6,7).
- Hai, T. et al. (1999) Gene Expr 7, 321-35.
- Hai, T. and Hartman, M.G. (2001) Gene 273, 1-11.
- Hai, T. et al. (2010) Gene Expr 15, 1-11.
- De Nardo, D. et al. (2014) Nat Immunol 15, 152-60.
- Kim, J. et al. (2014) J Biol Chem 289, 23246-55.
- Yin, X. et al. (2008) Oncogene 27, 2118-27.
- Wang, Z. et al. (2014) Oncogene Advanced online publication, doi:10.1038/onc.2014.426.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized) ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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