WB, IP
H
Endogenous
95, 98
Rabbit IgG
#Q9Y2T1
8313
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro566 of human Axin2 protein.
Background
Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice (6). Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).
- Luo, W. and Lin, S.C. Neurosignals 13, 99-113.
- Salahshor, S. and Woodgett, J.R. (2005) J. Clin. Pathol. 58, 225-236.
- Zeng, X. et al. (2005) Nature 438, 873-877.
- Davidson, G. et al. (2005) Nature 438, 867-872.
- Zhang, Y. et al. (1999) J. Biol. Chem. 274, 35247-35254.
- Chia, I.V. and Costantini, F. (2005) Mol. Cell Biol. 25, 4371-4376.
- Jho, E.H. et al. (2002) Mol. Cell Biol. 22, 1172-1183.
- Lustig, B. et al. (2002) Mol. Cell Biol. 22, 1184-1193.
- Leung, J.Y. et al. (2002) J. Biol. Chem. 277, 21657-21665.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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