WB, IP, IF-IC, FC-FP
Transfected Only
Mouse IgG1
#P00722
945006
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:3200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:7000 |
Storage
Specificity / Sensitivity
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant beta-galactosidase protein.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
Eukaryotic genes can be cloned into E. coli beta-galactosidase (lacZ) gene (1-3). This results in the expression of a fusion protein containing the desired protein with a beta-gal tag. An antibody to this tag can then be used to isolate the fusion protein or detect its expression within cells or tissues.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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