WB, IP
H M R
Endogenous
18
Rabbit IgG
#Q16143
6620
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human β-synuclein protein.
Background
The synuclein family, consisting of α-synuclein, β-synuclein, and γ-synuclein, are a group of small, soluble proteins containing a highly conserved α-helical lipid-binding motif. α-synuclein and β-synuclein are primarily expressed in brain tissue, where they colocalize within presynaptic terminals (1,2). α-synuclein is linked genetically and neuropathologically to Parkinson’s disease and other neurodegenerative diseases, called synucleinopathies, characterized by the presence of intracellular α-synuclein aggregates, the main component of Lewy bodies (3,4). β-synuclein has been shown to inhibit α-synuclein aggregation and ameliorate α-synuclein neurotoxicity (5-7). Studies have shown that β-synuclein levels are elevated in early and advanced Alzheimer’s disease, Creutzfeldt-Jakob disease, and traumatic brain injury, showing strong potential for use as a synaptic damage biomarker (8-9).
- George, J.M. (2002) Genome Biol 3, REVIEWS3002.
- Vigneswara, V. et al. (2013) PLoS One 8, e61442.
- Kim, W.S. et al. (2014) Alzheimers Res Ther 6, 73.
- Stefanis, L. (2012) Cold Spring Harb Perspect Med 2, a009399.
- Hashimoto, M. et al. (2001) Neuron 32, 213-23.
- Brown, J.W. et al. (2016) Sci Rep 6, 36010.
- Fan, Y. et al. (2006) Hum Mol Genet 15, 3002-11.
- Halbgebauer, S. et al. (2021) J Neurol Neurosurg Psychiatry 92, 349-356.
- Halbgebauer, R. et al. (2022) Int J Mol Sci 23, 9639. doi: 10.3390/ijms23179639.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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