IP, FC-FP, FC-L
H
Endogenous
Rabbit IgG
#Q68D85
374383
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunoprecipitation | 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
| Flow Cytometry (Live) | 1:50 - 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro185 of human B7-H6 protein.
Background
B7-H6 (NCR3LG1) is a member of the B7 family of proteins which provide costimulatory signals for regulating T cell responses (1-3). B7-H6 is a ligand for the NK-cell–activating receptor NKp30, which triggers anti-tumor natural killer (NK) cell cytotoxicity and cytokine secretion (1). Both forms of B7-H6, cell surface B7-H6 and soluble B7-H6 (sB7-H6), are detected in primary tumors and the tumor microenvironment, but rarely in normal tissues (1-5). Cell surface B7-H6 associates with cell damage, and triggers innate immunity when it binds to NKp30 on NK cells (3,5). Soluble B7-H6 can decrease NKp30 expression on NK cells and induce NK cell dysfunction (4,5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IP: Immunoprecipitation FC-FP: Flow Cytometry (Fixed/Permeabilized) FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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