Revision 4
#33059
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877-616-CELL (2355)
877-678-TECH (8324)
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, W-S, IP, ChIP, ChIP-seq
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
100
Source/Isotype:
Rabbit IgG
UniProt ID:
#O14867
Entrez-Gene Id:
571
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:100 |
| Chromatin IP-seq | 1:100 |
Storage
Specificity/Sensitivity
Source / Purification
Background
Background References
- Zhang, X. et al. (2018) Oxid Med Cell Longev 2018, 1347969.
- Oyake, T. et al. (1996) Mol Cell Biol 16, 6083-95.
- Dohi, Y. et al. (2008) Nat Struct Mol Biol 15, 1246-54.
- Ogawa, K. et al. (2001) EMBO J 20, 2835-43.
- Warnatz, H.J. et al. (2011) J Biol Chem 286, 23521-32.
- Nishizawa, H. et al. (2023) FEBS J 290, 1688-1704.
- Nishizawa, H. et al. (2020) J Biol Chem 295, 69-82.
- Igarashi, K. et al. (2021) J Biol Chem 297, 101032.
- Sun, J. et al. (2002) EMBO J 21, 5216-24.
- Dhakshinamoorthy, S. et al. (2005) J Biol Chem 280, 16891-900.
- Lee, J. et al. (2019) Nature 568, 254-258.
- Wiel, C. et al. (2019) Cell 178, 330-345.e22.
- Padilla, J. and Lee, J. (2021) Cells 10, 634.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting W-S: Simple Western™ IP: Immunoprecipitation ChIP: Chromatin IP ChIP-seq: Chromatin IP-seq
Cross-Reactivity Key
H: Human
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 4
Revision 4
Revision 4
