WB
H M R Mk
Endogenous
10, 11
Rabbit
#O75531
8815
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro14 of human BAF protein. Antibodies are purified by peptide affinity chromatography.
Background
Barrier-to-autointegration factor (BAF or BANF1) plays a major role in the lifecycle of retroviruses, where it inhibits the reverse transcribed viral genome from integrating into itself (1-3). BAF exists as a dimer in solution, and serves as a bridge for double-stranded DNA in an ordered nucleoprotein complex (4). BAF interacts with LEM-domains of lamin binding proteins emerin and LAP2, and serves to help reform the nuclear envelope during telophase (5-7). The binding of BAF to its various partners is inhibited by Ser4 phosphorylation, which is deposited by vaccinia-related kinases (VRKs) and occurs during interphase and mitosis (8,9). Certain viral genomes encode kinases extremely similar to VRK to prevent BAF from binding to their DNA, impeding viral replication (10,11). BAF has been implicated in various tumor types and is associated with poor prognosis (12-14).
- Lee, M.S. and Craigie, R. (1994) Proc Natl Acad Sci USA 91, 9823-7.
- Lee, M.S. and Craigie, R. (1998) Proc Natl Acad Sci USA 95, 1528-33.
- Cai, M. et al. (1998) Nat Struct Biol 5, 903-9.
- Zheng, R. et al. (2000) Proc Natl Acad Sci USA 97, 8997-9002.
- Shumaker, D.K. et al. (2001) EMBO J 20, 1754-64.
- Lee, K.K. et al. (2001) J Cell Sci 114, 4567-73.
- Haraguchi, T. et al. (2001) J Cell Sci 114, 4575-85.
- Bengtsson, L. and Wilson, K.L. (2006) Mol Biol Cell 17, 1154-63.
- Nichols, R.J. et al. (2006) Mol Biol Cell 17, 2451-64.
- Wiebe, M.S. and Traktman, P. (2007) Cell Host Microbe 1, 187-97.
- Jamin, A. et al. (2014) PLoS One 9, e100511.
- Li, J. et al. (2017) Biomed Pharmacother 89, 1086-1091.
- Shen, Q. and Nam, S.W. (2018) BMB Rep 51, 57-58.
- Li, J. et al. (2018) Oncol Lett 16, 6488-6494.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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