BAF Complex IHC Antibody Sampler Kit

1 Kit

(8 x 20 microliters)

Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

[email protected]

cellsignal.com

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes	Product #	Quantity	Mol. Wt	Isotype/Source
Brg1 (E8V5B) Mouse mAb	72182	20 µl	220 kDa	Mouse IgG1
PBRM1/BAF180 (D4L9X) Rabbit mAb	38439	20 µl	205 kDa	Rabbit IgG
SMARCB1/BAF47 (D8M1X) Rabbit mAb	91735	20 µl	44 kDa	Rabbit IgG
BRM (D9E8B) XP^® Rabbit mAb	11966	20 µl	200 kDa	Rabbit IgG
ARID1A/BAF250A (D2A8U) Rabbit mAb	12354	20 µl	270 kDa	Rabbit IgG
SS18-SSX (E9X9V) XP^® Rabbit mAb	72364	20 µl	65, 75 kDa	Rabbit IgG
ARID1B/BAF250B (E1U7D) Rabbit mAb	65747	20 µl	250, 280 kDa	Rabbit IgG
SS18 (D6I4Z) Rabbit mAb	21792	20 µl	Iso1 60, Iso2 50 kDa	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody	7074	100 µl		Goat
Anti-mouse IgG, HRP-linked Antibody	7076	100 µl		Horse

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The BAF Complex IHC Antibody Sampler Kit provides an economical means of detecting BAF complex members in formalin-fixed, paraffin-embedded tissue samples.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM (1). This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant (1). SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle, and differentiation (1). Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors (2). Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression, including HDACs, Rb, and Tif1β (3-5). Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also plays a role as a tumor suppressor and Brg1 is mutated in several tumor cell lines (6-8).

Background References

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

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Limited Uses

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com

For Research Use Only. Not for Use in Diagnostic Procedures.