Revision 2

#93589

Store at -20C

BAF Complex IHC Antibody Sampler Kit

1 Kit

(8 x 20 microliters)

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
Brg1 (E8V5B) Mouse mAb7218220 µl220 kDaMouse IgG1
PBRM1/BAF180 (D4L9X) Rabbit mAb3843920 µl205 kDaRabbit IgG
SMARCB1/BAF47 (D8M1X) Rabbit mAb9173520 µl44 kDaRabbit IgG
BRM (D9E8B) XP® Rabbit mAb1196620 µl200 kDaRabbit IgG
ARID1A/BAF250A (D2A8U) Rabbit mAb1235420 µl270 kDaRabbit IgG
SS18-SSX (E9X9V) XP® Rabbit mAb7236420 µl65, 75 kDaRabbit IgG
ARID1B/BAF250B (E1U7D) Rabbit mAb6574720 µl250, 280 kDaRabbit IgG
SS18 (D6I4Z) Rabbit mAb2179220 µlIso1 60, Iso2 50 kDaRabbit IgG
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat
Anti-mouse IgG, HRP-linked Antibody7076100 µlHorse

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The BAF Complex IHC Antibody Sampler Kit provides an economical means of detecting BAF complex members in formalin-fixed, paraffin-embedded tissue samples.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM (1). This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant (1). SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle, and differentiation (1). Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors (2). Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression, including HDACs, Rb, and Tif1β (3-5). Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also plays a role as a tumor suppressor and Brg1 is mutated in several tumor cell lines (6-8).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

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Western blot analysis of extracts from control HeLa cells (Lane 1) or HeLa cells with a targeted mutation in the gene encoding BRM (Lane 2) using BRM (D9E8B) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The change in BRM molecular weight in the mutated HeLa cells confirms the specificity of the antibody for BRM.
undefined Image 1: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using BRM (D9E8B) XP(R) Rabbit mAb.
undefined Image 2: BAF Complex IHC Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using ARID1A/BAF250A (D2A8U) Rabbit mAb.
undefined Image 3: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and ARID1A/BAF250A (D2A8U) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina (ChIP-seq, CUT&RUN) #56795. The figure shows binding across Oct-4/POU5F1, a known target gene of ARID1A (see additional figure containing ChIP-qPCR data).
undefined Image 4: BAF Complex IHC Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using SS18 (D6I4Z) Rabbit mAb.
undefined Image 5: BAF Complex IHC Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using PBRM1/BAF180 (D4L9X) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, HCC1143 cells are negative for PBRM1/BAF180 expression.
undefined Image 6: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using ARID1B/BAF250B (E1U7D) Rabbit mAb (upper) and α-Actinin (D6F6) Rabbit mAb #6487 (lower). MIA PaCa-2 cells do not express ARID1B/BAF250B protein.
undefined Image 7: BAF Complex IHC Antibody Sampler Kit
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
undefined Image 8: BAF Complex IHC Antibody Sampler Kit
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.
undefined Image 9: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using Brg1 (E8V5B) Mouse mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
undefined Image 10: BAF Complex IHC Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using SS18-SSX (E9X9V) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). ASKA is a synovial sarcoma cell line with an SS18-SSX1 fusion, while SYO-1 is a synovial sarcoma cell line with an SS18-SSX2 fusion. As shown, SS18-SSX (E9X9V) XP® Rabbit mAb only detects the fusion protein and not WT proteins. Cell lysates were isolated and provided by the Kadoch laboratory.
undefined Image 11: BAF Complex IHC Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using SMARCB1/BAF47 (D8M1X) Rabbit mab (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).
undefined Image 12: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using BRM (D9E8B) XP(R) Rabbit mAb.
undefined Image 13: BAF Complex IHC Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using BRM (D9E8B) XP® Rabbit mAb (upper) or Brg1 (A52) Antibody #3508 (lower).
undefined Image 14: BAF Complex IHC Antibody Sampler Kit
Western blot analysis of T-47D and Jurkat cell extracts using ARID1A/BAF250A (D2A8U) Rabbit mAb (upper) or ß-Actin (D6A8) Rabbit mAb #8457 (lower). Additional ARID1A/BAF250A degradation products may be detected in some cell extracts between 135kDa-250kDa, which are absent in the ARID1A/BAF250A negative T-47D cell line.
undefined Image 15: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and ARID1A/BAF250A (D2A8U) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina (ChIP-seq, CUT&RUN) #56795. The figure shows binding across chromosome 6 (upper), including Oct-4/POU5F1 (lower), a known target gene of ARID1A (see additional figure containing ChIP-qPCR data).
undefined Image 16: BAF Complex IHC Antibody Sampler Kit
Immunoprecipitation of SS18 from ACHN cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype control #3900, and lane 3 is SS18 (D6I4Z) Rabbit mAb. Western blot analysis was performed using SS18 (D6I4Z) Rabbit mAb.
undefined Image 17: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using PBRM1/BAF180 (D4L9X) Rabbit mAb performed on the Leica® BOND™ Rx.
undefined Image 18: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Brg1 (E8V5B) Mouse mAb.
undefined Image 19: BAF Complex IHC Antibody Sampler Kit
Immunoprecipitation of SS18-SSX (E9X9V) XP® Rabbit mAb from ASKA cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SS18-SSX (E9X9V) XP® Rabbit mAb. Western blot analysis was performed using SS18-SSX (E9X9V) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody. Cell lysates were isolated and provided by the Kadoch laboratory.
undefined Image 20: BAF Complex IHC Antibody Sampler Kit
Immunoprecipitation of SMARCB1/BAF47 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is SMARCB1/BAF47 (D8M1X) Rabbit mAb. Western blot analysis was performed usingSMARCB1/BAF47 (D8M1X) Rabbit mAb.
undefined Image 21: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human clear cell adenocarcinoma of the ovary using BRM (D9E8B) XP(R) Rabbit mAb.
undefined Image 22: BAF Complex IHC Antibody Sampler Kit
Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either ARID1A/BAF250A (D2A8U) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
undefined Image 23: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of mouse colon using ARID1A/BAF250A (D2A8U) Rabbit mAb.
undefined Image 24: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SS18 (D6I4Z) Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
undefined Image 25: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PBRM1/BAF180 (D4L9X) Rabbit mAb performed on the Leica® BOND™ Rx.
undefined Image 26: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded SH-SY5Y cell pellet (left, positive) or MIA PaCa-2 cell pellet (right, negative) using ARID1B/BAF250B (E1U7D) Rabbit mAb.
undefined Image 27: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung using Brg1 (E8V5B) Mouse mAb.
undefined Image 28: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human synovial sarcoma using SS18-SSX (E9X9V) XP® Rabbit mAb performed on the Leica® BOND™ Rx.
undefined Image 29: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SMARCB1/BAF47 (D8M1X) Rabbit mAb.
undefined Image 30: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded HeLa (left) and NCCIT (right) cell pellets using BRM (D9E8B) XP(R) Rabbit mAb.
undefined Image 31: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded cell pellets, COS-7 (left) or T-47D (right), using ARID1A/BAF250A (D2A8U) Rabbit mAb.
undefined Image 32: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using SS18 (D6I4Z) Rabbit mAb.
undefined Image 33: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

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Immunohistochemical analysis of paraffin-embedded normal human breast using PBRM1/BAF180 (D4L9X) Rabbit mAb.
undefined Image 34: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using ARID1B/BAF250B (E1U7D) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
undefined Image 35: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded SW620 cell pellet (left, positive) or A549 cell pellet (right, negative) using Brg1 (E8V5B) Mouse mAb.
undefined Image 36: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

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Immunohistochemical analysis of paraffin-embedded ASKA cell pellet (left, positive) or HeLa cell pellet (right, negative) using SS18-SSX (E9X9V) XP® Rabbit mAb. Cell pellets were isolated and provided by the Kadoch laboratory.
undefined Image 37: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded mouse colon using SMARCB1/BAF47 (D8M1X) Rabbit mAb.
undefined Image 38: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human lung adenosquamous carcinoma using ARID1A/BAF250A (D2A8U) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
undefined Image 39: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

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Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using SS18 (D6I4Z) Rabbit mAb.
undefined Image 40: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human colon using PBRM1/BAF180 (D4L9X) Rabbit mAb.
undefined Image 41: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ARID1B/BAF250B (E1U7D) Rabbit mAb.
undefined Image 42: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

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Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Brg1 (E8V5B) Mouse mAb.
undefined Image 43: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma (left) and adenoid cystic carcinoma of the trachea (right) using SS18-SSX (E9X9V) XP® Rabbit mAb (upper) or SS18 (D6I4Z) Rabbit mAb #21792 (lower). Note the absence of the fusion protein in each carcinoma.
undefined Image 44: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded endometrioid adenocarcinoma using SMARCB1/BAF47 (D8M1X) Rabbit mAb.
undefined Image 45: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using SS18 (D6I4Z) Rabbit mAb.
undefined Image 46: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the tonsil using PBRM1/BAF180 (D4L9X) Rabbit mAb.
undefined Image 47: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using ARID1B/BAF250B (E1U7D) Rabbit mAb.
undefined Image 48: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

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Immunohistochemical analysis of paraffin-embedded human synovial sarcoma using SS18-SSX (E9X9V) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
undefined Image 49: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded HeLa (left) and A204 (right) cell pellets using SMARCB1/BAF47 (D8M1X) Rabbit mAb.
undefined Image 50: BAF Complex IHC Antibody Sampler Kit
Confocal immunofluorescent analysis of HeLa (positive, left) and NCCIT (negative, right) cells using BRM (D9E8B) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).
undefined Image 51: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

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Immunohistochemical analysis of paraffin-embedded human synovial sarcoma using SS18 (D6I4Z) Rabbit mAb.
undefined Image 52: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using PBRM1/BAF180 (D4L9X) Rabbit mAb.
undefined Image 53: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ARID1B/BAF250B (E1U7D) Rabbit mAb.
undefined Image 54: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

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Immunohistochemical analysis of paraffin-embedded human synovial sarcoma (left) and normal testis (right) using SS18-SSX (E9X9V) XP® Rabbit mAb (upper) or SSX (E5A2C) Rabbit mAb (Carboxy-terminal Antigen) #23855 (lower). Note that the fusion protein is not present in normal testis.
undefined Image 55: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using SMARCB1/BAF47 (D8M1X) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
undefined Image 56: BAF Complex IHC Antibody Sampler Kit
Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d followed by treatment with β-estradiol (10 nM, 45 min) and either BRM (D9E8B) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
undefined Image 57: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

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Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d, followed by treatment with β-estradiol (10 nM, 45 min) and either SMARCC1/BAF155 (D7F8S) Rabbit mAb #11956, SMARCB1/BAF47 (D8M1X) Rabbit mAb #91735, or SS18 (D6I4Z) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. SMARCC1/BAF155, SMARCB1/BAF47, and SS18 are all subunits of SWI/SNF complex. The figure shows binding across pS2/TFF1, a known target gene of SWI/SNF complex (see additional figure containing ChIP-qPCR data).
undefined Image 58: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human granulosa cell tumor of the ovary using PBRM1/BAF180 (D4L9X) Rabbit mAb.
undefined Image 59: BAF Complex IHC Antibody Sampler Kit
Chromatin immunoprecipitations were performed with cross-linked chromatin from Aska cells and SS18-SSX (E9X9V) XP® Rabbit mAb, using SimpleChIP Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina #56795. The figure shows binding across HES5, a known target gene of SS18-SSX1 (see additional figure containing ChIP-qPCR data).
undefined Image 60: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d, followed by treatment with β-estradiol (10 nM, 45 min) and either SMARCC1/BAF155 (D7F8S) Rabbit mAb #11956, SMARCB1/BAF47 (D8M1X) Rabbit mAb #91735, or SS18 (D6I4Z) Rabbit mAb #21792, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. SMARCC1/BAF155, SMARCB1/BAF47, and SS18 are all subunits of SWI/SNF complex. The figure shows binding across pS2/TFF1, a known target gene of SWI/SNF complex (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.
undefined Image 61: BAF Complex IHC Antibody Sampler Kit
Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d, followed by treatment with β-estradiol (10 nM, 45 min) and either SMARCC1/BAF155 (D7F8S) Rabbit mAb #11956, SMARCB1/BAF47 (D8M1X) Rabbit mAb #91735, or SS18 (D6I4Z) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. SMARCC1/BAF155, SMARCB1/BAF47, and SS18 are all subunits of SWI/SNF complex. The figure shows binding across chromosome 21 (upper), including pS2/TFF1 (lower), a known target gene of SWI/SNF complex (see additional figure containing ChIP-qPCR data).
undefined Image 62: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded Jurkat cell pellet (left, positive) or HCC1143 cell pellet (right, negative) using PBRM1/BAF180 (D4L9X) Rabbit mAb.
undefined Image 63: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Chromatin immunoprecipitations were performed with cross-linked chromatin from Aska cells and SS18-SSX (E9X9V) XP® Rabbit mAb, using SimpleChIP Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina #56795. The figure shows binding across chromosome 1 (upper), including HES5 (lower), a known target gene of SS18-SSX1 (see additional figure containing ChIP-qPCR data).
undefined Image 64: BAF Complex IHC Antibody Sampler Kit
Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d, followed by treatment with β-estradiol (10 nM, 45 min) and either SMARCC1/BAF155 (D7F8S) Rabbit mAb #11956, SMARCB1/BAF47 (D8M1X) Rabbit mAb, or SS18 (D6I4Z) Rabbit mAb #21792, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. SMARCC1/BAF155, SMARCB1/BAF47, and SS18 are all subunits of SWI/SNF complex. The figure shows binding across chromosome 21 (upper), including pS2/TFF1 (lower), a known target gene of SWI/SNF complex (see additional figure containing ChIP-qPCR data).
undefined Image 65: BAF Complex IHC Antibody Sampler Kit
Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d then treated with β-estradiol (10 nM) for 45 min and either SS18 (D6I4Z) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
undefined Image 66: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

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Immunohistochemical analysis of paraffin-embedded normal human testis using PBRM1/BAF180 (D4L9X) Rabbit mAb.
undefined Image 67: BAF Complex IHC Antibody Sampler Kit
Chromatin immunoprecipitations were performed with cross-linked chromatin from ASKA cells and either SS18-SSX (E9X9V) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HES5 Upstream Primers #59885, human SOX8 exon 3 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. Chromatin pellets were isolated and provided by the Kadoch laboratory.
undefined Image 68: BAF Complex IHC Antibody Sampler Kit
Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d followed by treatment with β-estradiol (10 nM, 45 min) and either SMARCB1/BAF47 (D8M1X) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
undefined Image 69: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

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CUT&RUN was performed with MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d then treated with β-estradiol (10 nM) for 45 min and SS18 (D6I4Z) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across DEPTOR, a known target gene of SS18 (see additional figure containing CUT&RUN-qPCR data).
undefined Image 70: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human thymus using PBRM1/BAF180 (D4L9X) Rabbit mAb.
undefined Image 71: BAF Complex IHC Antibody Sampler Kit
CUT&RUN was performed with MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 days followed by treatment with β-estradiol (10 nM, 45 min) and SMARCB1/BAF47 (D8M1X) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA library was prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across TFF1, a known target gene of SMARCB1 (see additional figure containing CUT&RUN-qPCR data).
undefined Image 72: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

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Immunoprecipitation of BRM protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is BRM (D9E8B) XP® Rabbit mAb. Western blot analysis was performed using BRM (D9E8B) XP® Rabbit mAb.
undefined Image 73: BAF Complex IHC Antibody Sampler Kit
CUT&RUN was performed with MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d then treated with β-estradiol (10 nM) for 45 min and SS18 (D6I4Z) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 8 (upper), including DEPTOR (lower), a known target gene of SS18 (see additional figure containing CUT&RUN-qPCR data).
undefined Image 74: BAF Complex IHC Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human thyroid using PBRM1/BAF180 (D4L9X) Rabbit mAb.
undefined Image 75: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

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CUT&RUN was performed with MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 days followed by treatment with β-estradiol (10 nM, 45 min) and SMARCB1/BAF47 (D8M1X) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 21 (upper), including TFF1 (lower), a known target gene of SMARCB1 (see additional figure containing CUT&RUN-qPCR data).
undefined Image 76: BAF Complex IHC Antibody Sampler Kit
CUT&RUN was performed with MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d, followed by treatment with β-estradiol (10 nM, 45 min) and either SS18 (D6I4Z) Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human HMCN1 exon 96 primers, human TFF1/pS2 promoter primers, human DEPTOR intron 4 primers, and ITM2A upstream primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
undefined Image 77: BAF Complex IHC Antibody Sampler Kit
CUT&RUN was performed with MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 days followed by treatment with β-estradiol (10 nM, 45 min) and either SMARCB1/BAF47 (D8M1X) Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
undefined Image 78: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

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Simple Western™ analysis of lysates (1 mg/mL) from A549 cells using BRM (D9E8B) XP® Rabbit mAb #11966. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ ​​​​​​​ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
undefined Image 79: BAF Complex IHC Antibody Sampler Kit
Simple WesternTM analysis of lysates (0.1 mg/mL) from HS-SY-II using SS18-SSX (E9X9V) XP® Rabbit mAb #72364. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
undefined Image 80: BAF Complex IHC Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.