| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Brg1 (E8V5B) Mouse mAb | 72182 | 20 µl | 220 kDa | Mouse IgG1 |
| PBRM1/BAF180 (D4L9X) Rabbit mAb | 38439 | 20 µl | 205 kDa | Rabbit IgG |
| SMARCB1/BAF47 (D8M1X) Rabbit mAb | 91735 | 20 µl | 44 kDa | Rabbit IgG |
| BRM (D9E8B) XP® Rabbit mAb | 11966 | 20 µl | 200 kDa | Rabbit IgG |
| ARID1A/BAF250A (D2A8U) Rabbit mAb | 12354 | 20 µl | 270 kDa | Rabbit IgG |
| SS18-SSX (E9X9V) XP® Rabbit mAb | 72364 | 20 µl | 65, 75 kDa | Rabbit IgG |
| ARID1B/BAF250B (E1U7D) Rabbit mAb | 65747 | 20 µl | 250, 280 kDa | Rabbit IgG |
| SS18 (D6I4Z) Rabbit mAb | 21792 | 20 µl | Iso1 60, Iso2 50 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat | |
| Anti-mouse IgG, HRP-linked Antibody | 7076 | 100 µl | Horse |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The BAF Complex IHC Antibody Sampler Kit provides an economical means of detecting BAF complex members in formalin-fixed, paraffin-embedded tissue samples.
Background
The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM (1). This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant (1). SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle, and differentiation (1). Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors (2). Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression, including HDACs, Rb, and Tif1β (3-5). Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also plays a role as a tumor suppressor and Brg1 is mutated in several tumor cell lines (6-8).
- Trotter, K.W. and Archer, T.K. (2008) Nucl Recept Signal 6, e004.
- Trotter, K.W. and Archer, T.K. (2007) Mol Cell Endocrinol 265-266, 162-7.
- Sif, S. et al. (2001) Genes Dev 15, 603-18.
- Zhang, H.S. et al. (2000) Cell 101, 79-89.
- Underhill, C. et al. (2000) J Biol Chem 275, 40463-70.
- Magnani, L. and Cabot, R.A. (2009) Reproduction 137, 23-33.
- Medina, P.P. et al. (2008) Epigenetics 3, 64-8.
- Medina, P.P. et al. (2008) Hum Mutat 29, 617-22.
Background References
Trademarks and Patents
Limited Uses
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