WB, IP, FC-FP
H M
Endogenous
15
Rabbit IgG
#Q16520
10538
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #27977.
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BATF protein.
Background
Basic leucine zipper transcriptional factor ATF-like (BATF) is a basic leucine zipper (bZIP) transcription factor and is part of the AP-1/ATF family that forms inhibitory dimers with members of the Jun family (1-3). Expression of BATF is largely restricted with highest levels found in mature T cells, and it is induced in B cells following immune responses including viral infection (1,2). BATF expression is also induced by IL-6 via a Stat3-dependent mechanism (4). BATF plays an important role in the differentiation of immune cell lineages (5-7). Studies of BATF-deficient mice have demonstrated a critical role for BATF in the formation of IL-17-expressing Th17 cells, in part, by regulating the expression of IL-17 (5,6). BATF knockouts are resistant to experimental autoimmune encephalomyelitis (EEA), consistent with the role of Th17 cells in this model for autoimmunity (5). Additional studies have found that BATF is important in generating antibody class switching. BATF is required for the generation of follicular helper T cells (Tfh), by regulating BCL6 and c-Maf (6,7). In B cells, BATF controls the expression of activation-induced cytidine deaminase (AID) and regulates class-switched antibody responses (7). Taken together, these studies suggest that BATF is a key regulator of distinct populations of immune cells.
- Dorsey, M.J. et al. (1995) Oncogene 11, 2255-65.
- Hasegawa, H. et al. (1996) Biochem Biophys Res Commun 222, 164-70.
- Echlin, D.R. et al. (2000) Oncogene 19, 1752-63.
- Senga, T. et al. (2002) Oncogene 21, 8186-91.
- Schraml, B.U. et al. (2009) Nature 460, 405-9.
- Betz, B.C. et al. (2010) J Exp Med 207, 933-42.
- Ise, W. et al. (2011) Nat Immunol 12, 536-43.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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