WB, IP
H
Endogenous
105, 98, 85, 78
Rabbit IgG
#Q6ZUJ8
118788
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BCAP protein.
Background
B cell adaptor for PI3K (BCAP) is a multidomain-containing signaling adaptor that is expressed by myeloid cells, B cells, and NK cells (1,2). Two major BCAP protein isoforms that differ significantly in size, BCAP-L and BCAP-S, have been shown to be expressed by macrophages, B cells, and NK cells (1,2). Although it remains unclear if there is a differential function for these isoforms, both BCAP-L and BCAP-S undergo robust tyrosine phosphorylation by multiple tyrosine kinases in response to B cell receptor and Toll-like receptor engagement, which promotes association of BCAP with multiple SH2- and SH3-domain-containing proteins (1,3,4). In the context of B cells, research studies have demonstrated that phosphorylation of the YXXM motifs within BCAP are critical for the recruitment and activation of PI3K to drive B cell proliferation (4,5). Macrophages, however, utilize the BCAP/PI3K signaling module to restrain proinflammatory functions in response to infection (3,6).
- Okada, T. et al. (2000) Immunity 13, 817-27.
- MacFarlane, A.W. et al. (2008) Blood 112, 131-40.
- Ni, M. et al. (2012) Proc Natl Acad Sci U S A 109, 267-72.
- Inabe, K. and Kurosaki, T. (2002) Blood 99, 584-9.
- Yamazaki, T. et al. (2002) J Exp Med 195, 535-45.
- Troutman, T.D. et al. (2012) Proc Natl Acad Sci U S A 109, 273-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
