WB, IP
H M R
Endogenous
90
Rabbit IgG
#Q9BXK5
23786
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu267 of human BCL2L13 protein.
Background
BCL2L13 (Bcl-2-like protein 13, Bcl-rambo) is an atypical member of the Bcl-2 family of proteins that regulate apoptosis at the mitochondria (1). While it contains conserved Bcl-2 homology (BH) domains -1, -2, -3, and -4, it has a unique C-terminal region and does not interact with other Bcl-2 family members (1). BCL2L13 may regulate apoptosis in a cellular context manner (reviewed in 2). It was originally identified as a pro-apoptotic protein with activity dependent on its C-terminal region (1). Interaction with the adenine nucleotide translocator (ANT) suppresses the ADP/ATP-dependent translocation activity of ANT (3). Other studies have found that BCL2L13 can inhibit apoptosis in glioblastoma and tumor growth (4). These studies found that BCL2L13 binds to pro-apoptotic ceramide synthases 2 (CerS2) and 6 (CerS6) and blocks homo- and heteromeric CerS2/6 complex formation and activity. Studies have also found that BCL2L13 can regulate mitochondrial fragmentation and can bind to LC3 to facilitate the clearance of damaged mitochondria through mitophagy (5,6).
- Kataoka, T. et al. (2001) J Biol Chem 276, 19548-54.
- Kataoka, T. (2022) Front Cell Dev Biol 10, 1065702.
- Kim, J.Y. et al. (2012) FEBS Lett 586, 3142-9.
- Jensen, S.A. et al. (2014) Proc Natl Acad Sci USA 111, 5682-7.
- Murakawa, T. et al. (2015) Nat Commun 6, 7527.
- Otsu, K. et al. (2015) Autophagy 11, 1932-3.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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