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54831
PhosphoPlus® Beclin-1 (Ser30) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® Beclin-1 (Ser30) Antibody Duet #54831

Citations (1)
Western blot analysis of extracts from various cell lines using Beclin-1 (D40C5) Rabbit mAb.
Western blot analysis of extracts from HCT 116 cells, untreated (-) or treated with Torin 1 #14379 (250 nM, 2 hr; +) and/or the ULK1 inhibitor SBI-0206965 #29089 (50 μM, 2 hr; +) as indicated, using Phospho-Beclin-1 (Ser30) (E1C4X) Rabbit mAb (upper), Beclin-1 (D40C5) Rabbit mAb #3495 (middle), and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Beclin-1 siRNA I #6222 (+) or SignalSilence® Beclin-1 siRNA II (+), using Beclin-1 (D40C5) XP® Rabbit mAb #3495 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Beclin-1 (D40C5) XP® Rabbit mAb confirms silencing of Beclin-1 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Beclin-1 siRNA.
Western blot analysis of extracts from C2C12 and H-4-II-E cells, untreated (-) or treated with Torin 1 #14379 (250 nM, 2 hr; +), using Phospho-Beclin-1 (Ser30) (E1C4X) Rabbit mAb (upper), Beclin-1 (D40C5) Rabbit mAb #3495 (middle), and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human Beclin-1 protein (hBeclin-1-Myc/DDK; +) and/or mouse ULK1 protein (mULK1; +), using Phospho-Beclin-1 (Ser30) (E1C4X) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Immunoprecipitation of phospho-Beclin-1 (Ser30) from HCT 116 cell extracts treated with Torin 1 #14379 (250 nM, 2 hr). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-Beclin-1 (Ser30) (E1C4X) Rabbit mAb. Western blot analysis was performed using Phospho-Beclin-1 (Ser30) (E1C4X) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L29A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.
To Purchase # 54831
Cat. # Size Qty. Price
54831S
1 Kit

Product Includes Quantity Reactivity MW(kDa) Isotype
Phospho-Beclin-1 (Ser30) (E1C4X) Rabbit mAb 35955 100 µl H M R 60 Rabbit IgG
Beclin-1 (D40C5) Rabbit mAb 3495 100 µl H M R Mk 60 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL, but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, research studies have shown it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). Beclin-1 is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Investigators have demonstrated that Beclin-1-/- mice die early in embryogenesis and Beclin-1-/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response, although responses to apoptosis appeared normal (7). Researchers have also found that overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis (4).
ULK1 phosphorylates Beclin-1 at Ser30 to induce autophagy in response to amino acid starvation, mTORC1 inhibition, and hypoxia (8).

Pathways

Explore pathways related to this product.

Limited Uses

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