Revision 1

#85314

Store at -20C

beta-Amyloid Antibody Sampler Kit

1 Kit

(7 x 20 microliters)

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
APP Antibody245220 µl100 to 140 kDaRabbit
beta-Amyloid (1-42) (D3E10) Rabbit Monoclonal Antibody1284320 µl4 kDaRabbit
beta-Amyloid (1-40) (D8Q7I) Rabbit Monoclonal Antibody1299020 µl4 kDaRabbit IgG
beta-Amyloid (1-37) (D2A6H) Rabbit Monoclonal Antibody1246720 µl4 kDaRabbit IgG
beta-Amyloid (1-39) (D5Y9L) Rabbit Monoclonal Antibody1207720 µl4 kDaRabbit IgG
beta-Amyloid (D54D2) Rabbit Monoclonal Antibody824320 µl5 kDaRabbit IgG
beta-Amyloid (pE3 Peptide) (D5N5H) Rabbit Monoclonal Antibody1497520 µl4 kDaRabbit IgG
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The β-Amyloid Antibody Sampler Kit provides an economical means of detecting APP and APP unmodified/modified fragments using total and fragment-specific antibodies. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains an amyloid domain, which can be processed and released by two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). Several fragments corresponding to progressive APP processing at alternative cleavage sites have been identified (2). These include Aβ (1-37), Aβ (1-39), Aβ (1-40), and Aβ (1-42) (2). These fragments can also be N-terminally modified to generate pyroglutamate-3 Aβ (pE3-peptide) (3). Fragment-specific and pan-Aβ antibodies are used to detect and examine relative levels of individual Aβ fragments.

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of the indicated amounts of human Aβ-39 peptide using β-Amyloid (1-39) (D5Y9L) Rabbit mAb.
undefined Image 1: beta-Amyloid Antibody Sampler Kit
Western blot analysis of the indicated amounts of human Aβ-37 peptide using β-Amyloid (1-37) (D2A6H) Rabbit mAb.
undefined Image 2: beta-Amyloid Antibody Sampler Kit
Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42, and Aβ-43 peptides (2.5 ng) using β-Amyloid (1-42) (D3E10) Rabbit mAb (upper) or β-Amyloid (D54D2) XP® Rabbit mAb #8243 (lower).
undefined Image 3: beta-Amyloid Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of brain extracts from 13-month old wild-type and TG2576 mice using β-Amyloid (1-40) (D8Q7I) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
undefined Image 4: beta-Amyloid Antibody Sampler Kit
β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb specificity is demonstrated using peptide dot blot. β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb binds to pre-coated β-amyloid (pE3) peptide but not to β-amyloid (E3) peptide or scrambled β-amyloid (pE3) peptide.
undefined Image 5: beta-Amyloid Antibody Sampler Kit
Western blot analysis of extracts from HeLa (human), 3T3L1 (mouse) or C6 (rat) cells, untreated or nocodazole-treated (1 µg/ml, 16-18 hours), using APP Antibody.
undefined Image 6: beta-Amyloid Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
undefined Image 7: beta-Amyloid Antibody Sampler Kit
Western blot analysis of human Aβ-42, Aβ-40, Aβ-39, Aβ-38, and Aβ-37 peptides (5 ng) using β-Amyloid (D54D2) XP® Rabbit mAb.
undefined Image 8: beta-Amyloid Antibody Sampler Kit
Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42, and Aβ-43 peptides (10 ng) using β-Amyloid (1-39) (D5Y9L) Rabbit mAb (upper) or β-Amyloid (D54D2) XP® Rabbit mAb #8243 (lower).
undefined Image 9: beta-Amyloid Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of brain extracts from 13-month old wild-type and TG2576 mice using β-Amyloid (1-37) (D2A6H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
undefined Image 10: beta-Amyloid Antibody Sampler Kit
Western blot analysis of the indicated amounts of human Aβ-42 peptide using β-Amyloid (1-42) (D3E10) Rabbit mAb.
undefined Image 11: beta-Amyloid Antibody Sampler Kit
Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42 and Aβ-43 peptides (10 ng) using β-Amyloid (1-40) (D8Q7I) Rabbit mAb (upper) and β-Amyloid (D54D2) XP® Rabbit mAb #8243 (lower).
undefined Image 12: beta-Amyloid Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of the indicated amounts of human Aβ (pE3-42) and Aβ (1-42) peptides using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb (upper) and β-Amyloid (1-42) (D3E10) Rabbit mAb #12843 (lower).
undefined Image 13: beta-Amyloid Antibody Sampler Kit
Western blot analysis of the indicated amounts of human Aβ-42 (left) and Aβ-40 (right) peptides using β-Amyloid (D54D2) XP® Rabbit mAb.
undefined Image 14: beta-Amyloid Antibody Sampler Kit
Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42, and Aβ-43 peptides (10 ng) using β-Amyloid (1-37) (D2A6H) Rabbit mAb (upper) or β-Amyloid (D54D2) XP® Rabbit mAb #8243 (lower).
undefined Image 15: beta-Amyloid Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of brain extracts from 13-month old wild-type and TG2576 mice using β-Amyloid (1-42) (D3E10) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
undefined Image 16: beta-Amyloid Antibody Sampler Kit
Confocal immunofluorescent analysis of hippocampus from wild-type (left) and TG2576 (right) mice using β-Amyloid (1-40) (D8Q7I) Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 17: beta-Amyloid Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb.
undefined Image 18: beta-Amyloid Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of human Aβ-42 peptide (1 ng) and human cerebrospinal fluid (CSF) of an AD patient using β-Amyloid (D54D2) XP® Rabbit mAb.
undefined Image 19: beta-Amyloid Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded Tg2576 mouse model of Alzheimer's brain using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb.
undefined Image 20: beta-Amyloid Antibody Sampler Kit
Confocal immunofluorescent analysis of wild-type (left) and Tg2576 mouse model of Alzheimer's brain (right) using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 21: beta-Amyloid Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of mouse subicular cortex from an amyloid mouse model of Alzheimer's Disease using β-Amyloid (D54D2) XP® Rabbit mAb #8243 (green) and GFAP (GA5) Mouse mAb #3670 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 22: beta-Amyloid Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.