| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| BCAT1 (D6D4K) Rabbit mAb | 88785 | 20 µl | 43 kDa | Rabbit IgG |
| BCAT2 (D8K3O) Rabbit mAb | 79764 | 20 µl | 39 kDa | Rabbit IgG |
| BCKDH-E1α (E4T3D) Rabbit mAb | 90198 | 20 µl | 49 kDa | Rabbit IgG |
| Phospho-BCKDH-E1α (Ser293) (E2V6B) Rabbit mAb | 40368 | 20 µl | 49 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Branched-Chain Amino Acid Metabolism Antibody Sampler Kit provides an economical means of detecting select components involved in the branched-chain amino acid (BCAA) metabolism pathway. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
BCAT1 and BCAT2 are cytosolic and mitochondrial branched-chain aminotransferases, respectively (1,2). Research studies have implicated BCAT1 in distant metastasis in patients with advanced colorectal cancer (3). Disruption of BCAT2 in mice leads to higher levels of plasma branched-chain amino acids (BCAAs), reduced adiposity and body weight, and increased energy expenditure, suggesting its role in regulating insulin sensitivity (4). BCAAs leucine, isoleucine, and valine are essential amino acids in mammals, but elevated levels of BCAAs have been implicated in cardiovascular and metabolic disorders (5). The branched-chain α-keto acid dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the BCAA degradation pathway (6,7). Branched-chain α-keto acid decarboxylase (BCKDH-E1) is one of three enzymatic components in this complex (7). The α subunit of BCKDH-E1 (BCKDH-E1α) is critical for the regulation of BCKDH. Phosphorylation of BCKDH-E1α was shown to play a key role in regulating the enzymatic activity of this complex (7-9). Phosphorylation of BCKDH-E1α at Ser293 inactivates BCKDH (7,8). A significant elevation in plasma BCAA levels was reported to correlate with increased phosphorylation of BCKDH-E1α at Ser293 and suppressed BCKDH activity in the liver of diabetic mice (9).
- Bledsoe, R.K. et al. (1997) Biochim Biophys Acta 1339, 9-13.
- Suryawan, A. et al. (1998) Am J Clin Nutr 68, 72-81.
- Yoshikawa, R. et al. (2006) World J Gastroenterol 12, 5884-9.
- She, P. et al. (2007) Cell Metab 6, 181-94.
- Li, T. et al. (2017) Cell Metab 25, 374-385.
- Shin, A.C. et al. (2014) Cell Metab 20, 898-909.
- Lu, G. et al. (2009) J Clin Invest 119, 1678-87.
- Harris, R.A. et al. (1997) Adv Enzyme Regul 37, 271-93.
- Lian, K. et al. (2015) Diabetes 64, 49-59.
Background References
Trademarks and Patents
Limited Uses
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