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34995
Branched-Chain Amino Acid Metabolism Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Branched-Chain Amino Acid Metabolism Antibody Sampler Kit #34995

Citations (0)
Western blot analysis of extracts from various cell lines using Phospho-BCKDH-E1α (Ser293) (E2V6B) Rabbit mAb.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from MCF7, COS-7, and Vero cell lines using BCAT2 (D8K3O) Rabbit mAb.
Western blot analysis of Jurkat, MOLT-4, and Neuro-2a cells using BCAT1 (D6D4K) Rabbit mAb.
Western blot analysis of extracts from human liver, MCF7 and PANC-1 cell lines using BCKDH-E1α (E4T3D) Rabbit mAb.
Western blot analysis of extracts from HeLa cells, untreated (-) or treated (+) with calf intestinal alkaline phosphatase (CIP)/λ phosphatase, using Phospho-BCKDH-E1α (Ser293) (E2V6B) Rabbit mAb (upper) or BCKDH-E1α (E4T3D) Rabbit mAb #90198 (lower).
Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using BCAT2 (D8K3O) Rabbit mAb.
Immunoprecipitation of BCKDH-E1α from MCF7 extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is BCKDH-E1α (E4T3D) Rabbit mAb. Western blot analysis was performed using BCKDH-E1α (E4T3D) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.
Immunoprecipitation of Phospho-BCKDH-E1α (Ser293) from 293T extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-BCKDH-E1α (Ser293) (E2V6B) Rabbit mAb. Western blot analysis was performed using Phospho-BCKDH-E1α (Ser293) (E2V6B) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using BCAT2 (D8K3O) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using BCAT2 (D8K3O) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using BCAT2 (D8K3O) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded SCLC-21H cell pellet (left, high-expressing) or HepG2 cell pellet (right, low-expressing) using BCAT2 (D8K3O) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using BCAT2 (D8K3O) Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
To Purchase # 34995
Cat. # Size Qty. Price
34995T
1 Kit  (6 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
BCAT1 (D6D4K) Rabbit mAb 88785 20 µl
  • WB
H M 43 Rabbit IgG
BCAT2 (D8K3O) Rabbit mAb 79764 20 µl
  • WB
  • IP
  • IHC
H Mk 39 Rabbit IgG
BCKDH-E1α (E4T3D) Rabbit mAb 90198 20 µl
  • WB
  • IP
H M R 49 Rabbit IgG
Phospho-BCKDH-E1α (Ser293) (E2V6B) Rabbit mAb 40368 20 µl
  • WB
  • IP
H M R Mk 49 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Rab Goat 

Product Description

The Branched-Chain Amino Acid Metabolism Antibody Sampler Kit provides an economical means of detecting select components involved in the branched-chain amino acid (BCAA) metabolism pathway. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Branched-Chain Amino Acid Metabolism Antibody Sampler Kit detects endogenous levels of its target protein. BCAT1 (D6D4K) Rabbit mAb does not cross-react with BCAT2 protein. BCAT2 (D8K3O) Rabbit mAb does not cross-react with BCAT1 protein. Phospho-BCKDH-E1α (Ser293) (E2V6B) Rabbit mAb recognizes endogenous levels of BCKDH-E1α protein only when phosphorylated at Ser292 of mature human BCKDH-E1α, Ser293 of mature mouse BCKDH-E1α, or Ser293 of mature rat BCKDH-E1α.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the amino terminus of human BCAT1 protein, residues surrounding Leu39 of human BCAT2 protein, residues near the carboxy terminus of human BCKDH-E1α protein, and a synthetic phosphopeptide corresponding to residues surrounding Ser292 of mature human BCKDH-E1α protein, which corresponds to Ser293 of mature mouse BCKDH-E1α protein and Ser293 of mature rat BCKDH-E1α protein.

Background

BCAT1 and BCAT2 are cytosolic and mitochondrial branched-chain aminotransferases, respectively (1,2). Research studies have implicated BCAT1 in distant metastasis in patients with advanced colorectal cancer (3). Disruption of BCAT2 in mice leads to higher levels of plasma branched-chain amino acids (BCAAs), reduced adiposity and body weight, and increased energy expenditure, suggesting its role in regulating insulin sensitivity (4). BCAAs leucine, isoleucine, and valine are essential amino acids in mammals, but elevated levels of BCAAs have been implicated in cardiovascular and metabolic disorders (5). The branched-chain α-keto acid dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the BCAA degradation pathway (6,7). Branched-chain α-keto acid decarboxylase (BCKDH-E1) is one of three enzymatic components in this complex (7). The α subunit of BCKDH-E1 (BCKDH-E1α) is critical for the regulation of BCKDH. Phosphorylation of BCKDH-E1α was shown to play a key role in regulating the enzymatic activity of this complex (7-9). Phosphorylation of BCKDH-E1α at Ser293 inactivates BCKDH (7,8). A significant elevation in plasma BCAA levels was reported to correlate with increased phosphorylation of BCKDH-E1α at Ser293 and suppressed BCKDH activity in the liver of diabetic mice (9).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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