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9961
Cadherin-Catenin Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Cadherin-Catenin Antibody Sampler Kit #9961

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Cadherin-Catenin Antibody Sampler Kit: Image 1

Western blot analysis of extracts from HUVEC, NIH/3T3, MCF-7, ZR-75 and A431 cells using α-E-Catenin (23B2) Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 2

Immunohistochemical analysis of paraffin-embedded human colon using N-Cadherin (D4R1H) XP® Rabbit mAb. Note staining of myenteric plexus.

Cadherin-Catenin Antibody Sampler Kit: Image 3

Confocal immunofluorescent analysis of A172 (positive, left) and MCF7 (negative, right) cells using N-Cadherin (D4R1H) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

Cadherin-Catenin Antibody Sampler Kit: Image 4

Flow cytometric analysis of fixed and permeabilized HeLa cells (blue, negative) and MCF7 cells (green, positive) using E-Cadherin (24E10) Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 5

Confocal immunofluorescent analysis of A431 cells using P-Cadherin (C13F9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Cadherin-Catenin Antibody Sampler Kit: Image 6

Western blot analysis of extracts from various cell lines, using Pan-Cadherin (28E12) Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 7

Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT116 cells and either β-Catenin (D10A8) XP® Rabbit mAb or Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb #8814, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across AXIN2, a known target gene of β-Catenin (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.

Cadherin-Catenin Antibody Sampler Kit: Image 8

Confocal immunofluorescent analysis of HeLa (left) and NCI-H28 (right) cells using β-Catenin (D10A8) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Cadherin-Catenin Antibody Sampler Kit: Image 9

Western blot analysis of extracts from control HeLa cells (lane 1) or HeLa cells with an apparent in-frame truncation mutation in the gene encoding β-Catenin (lane 2) using β-Catenin (D10A8) XP® Rabbit mAb, #8480 (upper) or β-actin (D6A8) Rabbit mAb #8457 (lower). The change in β-Catenin molecular weight in the mutated HeLa cells is consistent with an in-frame deletion.

Cadherin-Catenin Antibody Sampler Kit: Image 10

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Cadherin-Catenin Antibody Sampler Kit: Image 11

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using N-Cadherin (D4R1H) XP® Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin’s Lymphoma using N-Cadherin (D4R1H) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Cadherin-Catenin Antibody Sampler Kit: Image 13

Confocal immunofluorescent images of MCF7 cells using E-Cadherin (24E10) Rabbit mAb (green, left) compared to an isotype control (right). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

Cadherin-Catenin Antibody Sampler Kit: Image 14

Western blot analysis of extracts from various cell types using P-Cadherin (C13F9) Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 15

Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT 116 cells and either β-Catenin (D10A8) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Axin2 Intron 1 Primers #8973, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Cadherin-Catenin Antibody Sampler Kit: Image 16

Immunohistochemical analysis of paraffin-embedded A172 (positive, left) and MCF7 (negative, right) cell pellets using N-Cadherin (D4R1H) XP® Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 17

Immunohistochemical analysis of paraffin-embedded human granulosa cell tumor of the ovary using N-Cadherin (D4R1H) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Cadherin-Catenin Antibody Sampler Kit: Image 18

Flow cytometric analysis of NCI-H28 cells (blue) and HeLa cells (green) using β-Catenin (D10A8) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as secondary antibody.

Cadherin-Catenin Antibody Sampler Kit: Image 19

Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using N-Cadherin (D4R1H) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Cadherin-Catenin Antibody Sampler Kit: Image 20

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using E-Cadherin (24E10) Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 21

Confocal immunofluorescent analysis of mouse colon using β-Catenin (D10A8) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Cadherin-Catenin Antibody Sampler Kit: Image 22

Immunohistochemical analysis of paraffin-embedded human metastatic adenocarcinoma in lymph node, using E-Cadherin (24E10) Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 23

Immunohistochemical analysis of paraffin-embedded mouse lung using E-Cadherin (24E10) Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 24

Immunohistochemical analysis of frozen mouse colon using β-Catenin (D10A8) XP® Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 25

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using E-Cadherin (24E10) Rabbit mAb in the presence of control peptide (left) or E-Cadherin Blocking Peptide #1050 (right).

Cadherin-Catenin Antibody Sampler Kit: Image 26

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Catenin (D10A8) XP® Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 27

Western blot analysis of extracts from A172 and MCF7 cells using N-Cadherin (D4R1H) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Cadherin-Catenin Antibody Sampler Kit: Image 28

Western blot analysis of extracts from various cell lines, using E-Cadherin (24E10) Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 29

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Catenin (D10A8) XP® Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 30

Immunohistochemical analysis of paraffin-embedded cell pellets, HeLa (left) or NCI-H28 (right), using β-Catenin (D10A8) XP® Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 31

Immunohistochemical analysis of paraffin-embedded mouse colon using β-Catenin (D10A8) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Cadherin-Catenin Antibody Sampler Kit: Image 32

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using ß-Catenin (D10A8) XP® Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 33

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using ß-Catenin (D10A8) XP® Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 34

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Catenin (D10A8) XP® Rabbit mAb.

Cadherin-Catenin Antibody Sampler Kit: Image 35

Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP® Rabbit mAb.

To Purchase # 9961T
Product # Size Price
9961T
1 Kit  (6 x 20 µl) $ 445

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
α-E-Catenin (23B2) Rabbit mAb 3240 20 µl
  • WB
  • IP
H M Mk 100 Rabbit IgG
N-Cadherin (D4R1H) XP® Rabbit mAb 13116 20 µl
  • WB
  • IP
  • IHC
  • IF
H M 140 Rabbit IgG
E-Cadherin (24E10) Rabbit mAb 3195 20 µl
  • WB
  • IHC
  • IF
  • F
H M 135 Rabbit IgG
P-Cadherin (C13F9) Rabbit mAb 2189 20 µl
  • WB
  • IF
H 120 Rabbit IgG
Pan-Cadherin (28E12) Rabbit mAb 4073 20 µl
  • WB
H M R 130-150 Rabbit IgG
β-Catenin (D10A8) XP® Rabbit mAb 8480 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
  • ChIP
H M R Mk 92 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

This Cadherin-Catenin Antibody Sampler kit contains reagents to examine the total protein levels of key proteins found in cell-cell adherens junctions. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.

Specificity / Sensitivity

Each antibody in the Cadherin-Catenin Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Pan-Cadherin (28E12) Rabbit mAb #4073 detects endogenous levels of total cadherin proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human α-E-catenin, residues surrounding Arg526 of human N-cadherin protein, residues near the carboxy terminus of human P-cadherin, residues surrounding 780 of human E-cadherin, residues surrounding Pro714 of human ß-catenin protein, and a synthetic peptide corresponding to a conserved region of human N-, R-, E- and P-Cadherin.

Background

Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of cadherins that are transmembrane proteins that bind cadherins on adjacent cells in a calcium dependent manner. On the cytoplasmic side of adherens junctions, the cadherins associate with β-catenin, γ-catenin and p120 catenin (δ). β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Recent studies indicate that cancer cells exhibit increased N-cadherin and diminished E-cadherin expression. E-cadherin is considered a suppressor of invasive cancer cell growth and this change in cadherin expression associated with cancer progression is termed the “cadherin switch”. β-catenin is one of the key downstream effectors in the Wnt signaling pathway and has been implicated in early embryonic development and tumorigenesis (3-5).

  1. Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35.
  2. Christofori, G. (2003) EMBO J 22, 2318-23.
  3. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-3305.
  4. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  5. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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