WB, IHC-P, IF-F, IF-IC
H M R
Endogenous
29
Rabbit IgG
#P22676
794
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
| Immunofluorescence (Frozen) | 1:100 - 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #87716.
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Calretinin protein.
Background
Calretinin (29 kDa calbindin, calbindin 2) is a calcium-binding protein of the EF-hand family encoded by the CALB2 gene. It is differentially expressed from homologous family member calbindin-d28k in distinct neuronal populations of the retina, auditory system, and cerebellar granule cells (1,2), and acts as a marker for specific neuronal subsets of the subthalamic nucleus and the substantia nigra (3). Calretinin has been shown to play an important role in modulating neuronal excitability and the induction of long-term potentiation (1). Research has shown that, pathologically, Calretinin is a selective marker for epithelial mesothelioma, making it a diagnostic tool to differentiate from adenocarcinomas (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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