WB, IP
H M R Hm Mk
Endogenous
32
Rabbit IgG
#P49069
819
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu110 of human CAMLG protein.
Background
The calcium signal-modulating cyclophilin ligand (CAMLG) is a multi-pass endoplasmic reticulum (ER) membrane protein that plays an important role in calcium-mediated signal transduction. The CAMLG protein was first identified in T lymphocytes as a cyclophilin B-binding protein that regulates calcium influx following T-cell receptor (TCR) activation (1). Research studies indicate that CAMLG overexpression activates the transcription factors NFAT and NF-IL2A and leads to increased IL-2 gene transcription, providing additional evidence that CAMLG plays an important role in TCR signal transduction. CAMLG negatively regulates the non-receptor protein kinase Lck, which is critical for the thymocyte selection process (2). Thymocytes deficient for CAMLG fail to undergo normal development, accumulate reactive oxygen species, and exhibit increased apoptosis in response to cytotoxic stimuli (3). The CAMLG protein also acts as a receptor of TRC40, an ATPase that targets newly synthesized proteins in the secretory pathway to the ER membrane. CAMLG interacts with the protein insertion receptor WRB to form the TRC40 receptor complex that is responsible for insertion of tail-anchored proteins into the ER membrane (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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