IP, IF-F
M
Endogenous
15
Rabbit IgG
#Q16568
9607
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Frozen) | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human CART protein.
Background
Cocaine- and amphetamine-regulated transcript (CART) peptides are neurotransmitters of 39 and 47 amino acids that are involved in a variety of physiological processes. The CART precursor, a polypeptide of 116 residues, requires prohormone/proprotein convertase-mediated endoproteolytic cleavage to produce the two active peptides (1). CART peptides are found in several neuroendocrine tissues such as the brain, pituitary, adrenals, and pancreas (2). Hypothalamic CART is regulated by leptin, and plays a role in appetite and feeding behavior (3). Mesolimbic CART is regulated by CREB and may play a role in drug abuse behaviors by mediating some of CREB effects (4). Pancreatic CART is found in islet endocrine cells and parasympathetic and sensory nerves. It inhibits glucose-stimulated insulin secretion and has been found to be up-regulated in beta cells in animal model of diabetes (5). A missense mutation in the corresponding CART gene can correlate with susceptibility to obesity and reduced resting energy expenditure (6).
- Stein, J. et al. (2006) Peptides 27, 1919-25.
- Hunter, R.G. and Kuhar, M.J. (2003) Curr Drug Targets CNS Neurol Disord 2, 201-5.
- Thim, L. et al. (1998) Int J Biochem Cell Biol 30, 1281-4.
- Kuhar, M.J. et al. (2005) AAPS J 7, E259-65.
- Wierup, N. and Sundler, F. (2006) Peptides 27, 2031-6.
- del Giudice, E.M. et al. (2001) Diabetes 50, 2157-60.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IP: Immunoprecipitation IF-F: Immunofluorescence (Frozen)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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