| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Cas9 (S. pyogenes) (D8Y4K) Rabbit mAb | 65832 | 20 µl | 150 kDa | Rabbit IgG |
| Cas9 (S. aureus) (E4G3U) Rabbit mAb | 51610 | 20 µl | 124 kDa | Rabbit IgG |
| AsCpf1/Cas12a (Strain BV3L6) (E1U7C) Rabbit mAb | 19984 | 20 µl | 151 kDa | Rabbit IgG |
| FnCpf1/Cas12a (Strain U112) (E7I2B) Rabbit mAb | 90111 | 20 µl | 152 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Cas9 and Associated Proteins Antibody Sampler Kit provides an economical means of detecting Cas9 and Cas9-related family members. The kit includes enough antibody to perform two western blot experiments with each primary antibody.
Storage
Background
CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Class 2 CRISPR systems rely on single-component effector proteins to mediate DNA interference (2). Several Class 2 CRISPR effector proteins, derived from specific bacterial species, are used for genome editing. Cas9 family of proteins, derived from S. pyogenes and S. aureus, are some of the most well characterized and widely used editing effector enzymes. Additional members of the Class2 CRISPR system include Cpf1/Cas12a (CRISPR from Prevotella and Francisella) endonucleases (3). Cpf1/Cas12a endonucleases, compared to Cas9 systems, have several unique features that increase the utility of CRISPR-based genome editing techniques: 1) Cpf1/Cas12a-mediated cleavage relies on a single and short CRISPR RNA (crRNA) without the requirement of a trans-activating crRNA (tracrRNA), 2) Cpf1/Cas12a utilizes T-Rich protospacer adjacent motif (PAM) sequences rather than a G-Rich PAM, and 3) Cpf1/Cas12a generates a staggered, rather than a blunt-ended, DNA double-stranded break (3). These features broaden the utility of using CRISPR-Cas systems for specific gene regulation and therapeutic applications. Several Cpf1/Cas12a bacterial orthologs, e.g. Francisella novicida U112 and Acidaminococcus sp. BV3L6, have been characterized for CRISPR-mediated mammalian genome editing (3,4).
Background References
Trademarks and Patents
Limited Uses
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