WB, IP
M
Endogenous
38, 43
Rat IgG2a
#P70343
12363
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the p30 subunit terminus of mouse caspase-11 protein.
Background
Caspases are a family of aspartate-specific cysteine-dependent proteases that play a critical role in apoptosis as well as inflammatory responses. Pro-inflammatory caspases include caspase-1 and mouse caspase-11 (1). Caspase-11 has about 60% and 55% identity to human caspases-4 and -5, respectively. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1β and IL-18 into their mature forms (2). It is activated by proteolytic cleavage producing a tetramer of its two active subunits, p20 and p10. Canonical activation of caspase-1 occurs through several complex molecular platforms designated “inflammasomes” that include Pycard/Asc, nucleotide-binding oligomerization receptors (NLRs), and AIM2 (3, 4). Non-canonical activation of caspase-1 is triggered by caspase-11, which is transcriptionally induced by toll-like receptor ligands including LPS. Activation of this pathway induces inflammatory cytokines as well as pyroptosis, a form of programmed cell death (5-9). Pyroptosis may be specific for caspase-11, rather than caspase-1, suggesting a unique mechanism for protease.
- Ng, T.M. and Monack, D.M. (2013) Cell Host Microbe 14, 9-14.
- Thornberry, N.A. et al. (1992) Nature 356, 768-74.
- Martinon, F. et al. (2002) Mol Cell 10, 417-26.
- Lamkanfi, M. and Dixit, V.M. (2014) Cell 157, 1013-22.
- Hagar, J.A. et al. (2013) Science 341, 1250-3.
- Kayagaki, N. et al. (2013) Science 341, 1246-9.
- Aachoui, Y. et al. (2013) Science 339, 975-8.
- Broz, P. et al. (2012) Nature 490, 288-91.
- Kayagaki, N. et al. (2011) Nature 479, 117-21.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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