WB, IP
H
Endogenous
22-25, 32-48
Rabbit IgG
#P49662
837
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the p20 subunit of human caspase-4 protein.
Background
Caspase-4 (TX/ICH-2/ICErelII) is a member of the caspase family of proteases that play a key role in the execution of apoptosis and activation of inflammatory cytokines (1-3). Expression of caspase-4 has been observed in most tissues except brain, with highest levels in placenta, lung, spleen, and peripheral blood lymphocytes (PBL). Caspase-4 was originally found to contribute to Fas-mediated apoptosis (4). Several caspases (including caspase-4, caspase-5, and mouse caspase-11 and -12) are most closely related to caspase-1 and are capable of inducing apoptosis when overexpressed but are better characterized in the proteolytic activation of inflammatory cytokines (5). Caspase-4 associates with TRAF6 and is involved in the LPS inducible production of inflammatory cytokines IL-8 and MIP1 in THP-1 cells (6). While caspase-4 and mouse caspase-12 localize to the endoplasmic reticulum (ER) and may be activated by drugs that induce ER stress (7), at least one study suggests that caspase-4 and caspase-12 are not essential for ER stress-induced apoptosis (8).
- Faucheu, C. et al. (1995) EMBO J 14, 1914-22.
- Kamens, J. et al. (1995) J Biol Chem 270, 15250-6.
- Munday, N.A. et al. (1995) J Biol Chem 270, 15870-6.
- Kamada, S. et al. (1997) Oncogene 15, 285-90.
- Martinon, F. and Tschopp, J. (2007) Cell Death Differ 14, 10-22.
- Lakshmanan, U. and Porter, A.G. (2007) J Immunol 179, 8480-90.
- Hitomi, J. et al. (2004) J Cell Biol 165, 347-56.
- Obeng, E.A. and Boise, L.H. (2005) J Biol Chem 280, 29578-87.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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