Revision 5

#74089

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, W-S, IHC-P, IF-F, IF-IC

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
46, 43, 28

Source/Isotype:
Rabbit IgG

UniProt ID:
#P18242

Entrez-Gene Id:
13033

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:50 - 1:250
Immunohistochemistry (Paraffin) 1:75 - 1:300
Immunofluorescence (Frozen) 1:800 - 1:1600
Immunofluorescence (Immunocytochemistry) 1:400 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Cathepsin D (E5V4H) Rabbit Monoclonal Antibody detects endogenous levels of preprocathepsin D, procathepsin D, and the heavy chain subunit of mature cathepsin D protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu179 of mouse cathepsin D protein.

Background

Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularly in breast cancer (4-9).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting W-S: Simple Western™ IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Western blot analysis of extracts from various brain tissues and HCC1419 cells using Cathepsin D (E5V4H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 1: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Simple Western™ analysis of lysates (0.5 mg/ml) from mouse brain lysates using Cathepsin D (E5V4H) Rabbit mAb #74089. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230kDa.
Western Blotting Image 1: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the cervix using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemistry Image 1: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human papillary thyroid carcinoma using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemistry Image 2: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemistry Image 3: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human adrenal gland using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemistry Image 4: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human pancreas using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemistry Image 5: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human lymph node using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemistry Image 6: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human brain using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemistry Image 7: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded mouse pancreas using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemistry Image 8: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody


Immunohistochemical analysis of paraffin-embedded mouse thymus using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemistry Image 9: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse testes using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemistry Image 10: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma (left) and tonsil (right) using Cathepsin D (E5V4H) Rabbit mAb (top) or a Cathepsin D Rabbit pAb (bottom). These two antibodies detect unique, non-overlapping epitopes on human cathepsin D. The similar patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemistry Image 11: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse cerebellum (left) and A20 syngeneic tumor (right) using Cathepsin D (E5V4H) Rabbit mAb (top) or a Cathepsin D Rabbit mAb (bottom). These two antibodies detect unique, non-overlapping epitopes on mouse cathepsin D. The similar patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemistry Image 12: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human liver using Cathepsin D (E5V4H) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). 
Immunohistochemistry Image 13: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded U-118 MG cell pellet (left, positive) or Ramos cell pellet (right, negative) using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemistry Image 14: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen brain from an amyloid mouse model of Alzheimer's disease using Cathepsin D (E5V4H) Rabbit mAb (left, green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (right, red) and β-Amyloid (D3D2N) Mouse mAb #15126 (right, blue).
Immunofluorescence Image 1: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen mouse brain using Cathepsin D (E5V4H) Rabbit mAb (left, green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (right, red) and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 2: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of fixed frozen mouse colon at high magnification using Cathepsin D (E5V4H) Rabbit mAb (left, green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (right, red) and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 3: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen mouse colon at low magnification using Cathepsin D (E5V4H) Rabbit mAb (left, green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (right, red) and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 4: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of HCC1419 cells (left) and U-118 MG cells (right) using Cathepsin D (E5V4H) Rabbit mAb (green), β-Actin (8H10D10) Mouse mAb #3700 (red), and DAPI #4083 (blue).
Immunofluorescence Image 1: Cathepsin D (E5V4H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.