WB, IP, IHC-Bond, IHC-P
H M
Endogenous
190
Rabbit IgG
#Q6YHK3
135228
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| IHC Leica Bond | 1:400 - 1:1600 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #34464.
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val834 of human CD109 protein.
Background
CD109 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein that belongs to the alpha2-macroglobulin family of thioester containing proteins (1). CD109 is associated with TGF-beta receptor I (TbRI) and inhibits TGF-beta signaling (2,3). Cleavage of CD109 at its Furin cleavage site results in the release of its large amino-terminal domain, which then binds to the TGF-beta receptor I to inhibit TGF-beta signaling (4-7). CD109 is expressed on a subset of CD34+ bone marrow cells and mesenchymal stem cells, activated platelets, activated T cells, endothelial cells, and a wide variety of tumors (8-10). Elevated CD109 expression has been considered a diagnostic/prognostic marker for several types of cancers (11-14).
- Lin, M. et al. (2002) Blood 99, 1683-91.
- Finnson, K.W. et al. (2006) FASEB J 20, 1525-7.
- Zhang, J.M. et al. (2015) Biochem Biophys Res Commun 459, 252-8.
- Hagiwara, S. et al. (2010) Oncogene 29, 2181-91.
- Litvinov, I.V. et al. (2011) Exp Dermatol 20, 627-32.
- Zhou, S. et al. (2017) Oncotarget 8, 95632-47.
- Li, C. et al. (2016) Biochem J 473, 537-47.
- Hashimoto, M. et al. (2004) Oncogene 23, 3716-20.
- Sato, T. et al. (2007) Pathol Int 57, 719-24.
- Qi, R. et al. (2018) J Transl Med 16, 88.
- Emori, M. et al. (2015) J Surg Oncol 111, 975-9.
- Jia, W. et al. (2016) Oncotarget 7, 55328-42.
- Yokoyama, M. et al. (2017) Int J Hematol 105, 614-22.
- Chuang, C.H. et al. (2017) Nat Med 23, 291-300.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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